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Invitrogen
AXIN2 Monoclonal Antibody (J.415.10)
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FIGURE: 1 / 5
AXIN2 Antibody (MA5-15015) in WB
Western blot was performed using Anti-AXIN2 Monoclonal Antibody (J.415.10)(Product # MA5-15015) and a 94 kDa band corresponding to AXIN2 was observed across tissues tested. Tissue extracts (30 µg lysate) of Rat Brain (Lane 1), Rat Prostate (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
J.415.10
Immunogen
Synthetic peptide corresponding to residues surrounding Pro566 of human Axin2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
183 µg/mL
Amount
18.3 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with Axin1.
Target Information
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Axin like protein; axin-like protein; AXIN2; axis inhibition protein 2; conductin; DKFZp781B0869; MGC10366; MGC126582
Gene Aliases: AXIL; ODCRCS
UniProt ID: (Human) Q9Y2T1
Entrez Gene ID: (Human) 8313
aortic valve morphogenesis
mitral valve morphogenesis
protein localization
negative regulation of cell proliferation
dorsal/ventral axis specification
positive regulation of epithelial to mesenchymal transition
negative regulation of Wnt signaling pathway
regulation of mismatch repair
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
odontogenesis
proteasome-mediated ubiquitin-dependent protein catabolic process
maintenance of DNA repeat elements
positive regulation of fat cell differentiation
mRNA stabilization
cell development
response to steroid hormone
canonical Wnt signaling pathway
regulation of centromeric sister chromatid cohesion
cellular response to dexamethasone stimulus
negative regulation of canonical Wnt signaling pathway
regulation of extracellular matrix organization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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