Western blot was performed using Anti-AXIN2 Monoclonal Antibody (J.415.10)(Product # MA5-15015) and a 94 kDa band corresponding to AXIN2 was observed across tissues tested. Tissue extracts (30 µg lysate) of Rat Brain (Lane 1), Rat Prostate (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and ... View More

Product Image Gallery

AXIN2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Rat Brain and Rat Prostate owing to their inherent genetic constitution. Relative expression of AXIN2 was observed in Rat Brain (High expressing) and Rat Prostate (Low expressing), as reported, using Anti-AXIN2 Monoclonal Antibody (J.415.10) (Product # MA5-15015) in Western Blot. {RE}

Immunogen image

Immunogen Image AXIN2 Monoclonal Antibody (J.415.10)

Product Details

MA5-15015

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

J.415.10

Immunogen

Synthetic peptide corresponding to residues surrounding Pro566 of human Axin2

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

183 µg/mL

18.3 µg

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10984109

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with Axin1.

Target Information

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Axil; Axin like protein; Axin-2; Axin-like protein; AXIN2; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582

View more View less

Gene Aliases: AXIL; AXIN2; ODCRCS

View more View less

UniProt ID: (Human) Q9Y2T1

View more View less

Entrez Gene ID: (Human) 8313

View more View less

Function(s)

protein binding beta-catenin binding enzyme binding protein kinase binding ubiquitin protein ligase binding binding, bridging I-SMAD binding

Process(es)

osteoblast differentiation somitogenesis intramembranous ossification secondary heart field specification aortic valve morphogenesis mitral valve morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis protein localization cell proliferation negative regulation of cell proliferation dorsal/ventral axis specification positive regulation of epithelial to mesenchymal transition Wnt signaling pathway regulation of Wnt signaling pathway negative regulation of Wnt signaling pathway bone mineralization regulation of mismatch repair positive regulation of proteasomal ubiquitin-dependent protein catabolic process osteoblast proliferation negative regulation of osteoblast proliferation odontogenesis proteasome-mediated ubiquitin-dependent protein catabolic process maintenance of DNA repeat elements positive regulation of fat cell differentiation negative regulation of osteoblast differentiation mRNA stabilization cell development response to steroid hormone canonical Wnt signaling pathway regulation of chondrocyte development regulation of centromeric sister chromatid cohesion cellular response to dexamethasone stimulus stem cell proliferation negative regulation of canonical Wnt signaling pathway regulation of extracellular matrix organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

AXIN2 Monoclonal Antibody (J.415.10)

AXIN2 Antibody (MA5-15015) in WB

Product Details

MA5-15015

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!