Search
Invitrogen
HDGF Recombinant Rabbit Monoclonal Antibody (JE56-32)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 10
HDGF Antibody (MA5-42522) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of HDGF in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HDGF Recombinant Monoclonal Antibody (Product # MA5-42522) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Product Details
MA5-42522
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE56-32
Immunogen
Recombinant protein within human HDGF aa 50-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Rat cerebellum tissue lysate, SK-Br-3 cell lysate, A549 cell lysate, mouse cerebellum tissue lysate, F9, human liver carcinoma tissue, human cervix tissue, human colon carcinoma tissue, human breast carcinoma tissue, human esophagus tissue, human stomach carcinoma tissue, human small intestine tissue, THP-1.
Subcellular Location: Nucleus, Cytoplasm, Extracellular exosome.
Target Information
HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DKFZp686J1764; epididymis secretory sperm binding protein; FLJ96580; HDGF; hepatoma derived growth factor; Hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2; unnamed protein product
Gene Aliases: AI118077; D3Ertd299e; HDGF; HMG1L2; Tdrm1
UniProt ID: (Human) P51858, (Rat) Q8VHK7, (Mouse) P51859
Entrez Gene ID: (Human) 3068, (Rat) 114499, (Mouse) 15191
nucleotide binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription corepressor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription corepressor activity
RNA binding
actin binding
protein binding
growth factor activity
heparin binding
tubulin binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription corepressor activity
poly(A) RNA binding
transcription cofactor
negative regulation of transcription from RNA polymerase II promoter
chromatin remodeling
signal transduction
protein localization to nucleus
positive regulation of transcription from RNA polymerase II promoter
positive regulation of cell division
transcription, DNA-templated
cellular process
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support