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Invitrogen
Phospho-IRF7 (Ser471, Ser472) Polyclonal Antibody
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FIGURE: 1 / 6
Phospho-IRF7 (Ser471, Ser472) Antibody (PA5-114592) in ICC/IF
Immunocytochemistry analysis of Phospho-IRF7 (Ser471, Ser472) in HeLa cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114592). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 48... View More
Product Details
PA5-114592
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human IRF7 around the phosphorylation site of Ser471/472.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of IRF7 only when phosphorylated at Ser471/472.
Target Information
Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7; IRF-7H; unnamed protein product
Gene Aliases: IMD39; IRF-7; IRF-7H; IRF7; IRF7A; IRF7B; IRF7C; IRF7H
UniProt ID: (Human) Q92985, (Mouse) P70434
Entrez Gene ID: (Human) 3665, (Mouse) 54123, (Rat) 293624
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
regulatory region DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
immune system process
cytoplasmic pattern recognition receptor signaling pathway
regulation of adaptive immune response
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
signal transduction
response to virus
regulation of gene expression
immunoglobulin mediated immune response
establishment of viral latency
regulation of type I interferon production
positive regulation of type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
regulation of MyD88-dependent toll-like receptor signaling pathway
regulation of MyD88-independent toll-like receptor signaling pathway
MDA-5 signaling pathway
innate immune response
positive regulation of innate immune response
regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of immune response
defense response to virus
type I interferon signaling pathway
positive regulation of type I interferon-mediated signaling pathway
negative regulation of macrophage apoptotic process
transcription, DNA-templated
transcription from RNA polymerase II promoter
interferon-alpha production
interferon-beta production
regulation of innate immune response
type I interferon biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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