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PeproGMP® Human VEGF-165 Recombinant Protein, PeproTech®
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FIGURE: 1 / 1
PeproGMP® Human VEGF-165 Protein (GMP100-20-100UG) in Bioactivity
Cell proliferation assay analysis of PeproGMP® Human VEGF-165 Recombinant Protein, PeproTech (Product # GMP100-20-50UG) compared to Human VEGF-165 Recombinant Protein, PeproTech (Product # 100-20-1MG), demonstrating comparable bioactivity across product grades.
Product Details
GMP100-20-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Molecular weight
38.2 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
≤0.1 EU/µg
Activity
Determined by the proliferation of HUVECs.
Conjugate
Form
Lyophilized
Amount
100 µg
Purification
purified
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
PeproTech Recombinant Human VEGF-165 is a 38.2 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
PeproGMP VEGF-165 can be used with PeproGMP BMP-4 and PeproGMP SCF in CTS StemScale medium for pluripotent stem cell differentiation, specifically for mesoderm and hematopoetic progenitor cell induction.
Reconstitute using sterile water (i.e., WFI) to a concentration of 0.1-1.0 mg/ml.
Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis.
All PeproGMP® products are manufactured using animal-derived component free materials.
Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
Target Information
The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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Bioinformatics
Protein Aliases: growth factor; H-VEGF-165; L-VEGF; M-VEGF-165; vascular endothelial growth factor; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; vascular permeability factor precursor; VEGF121; VEGF165; VPF
Gene Aliases: L-VEGF; MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) P15692
Entrez Gene ID: (Human) 7422
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