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Invitrogen
IL-6 Recombinant Protein
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FIGURE: 1 / 1
IL-6 Protein (SIL6) in ELISA Std
Sandwich ELISA analysis of human IL-6 was performed using a Human IL-6 Colorimetric ELISA kit (Product # EH2IL6) by loading 50 ul per well of a mouse anti-human IL-6 biotinylated antibody (Product # M621B) at a concentration of 0.035 ug/ml and 50 ul of Human IL-6 Recombinant Protein (Product # SIL6) at 400, 160, 64, 25.6, 10.24, and 0 pg/ml (both in quadruplicate) across a 4 ug/ml mouse anti-human IL-6 (Product # M620) pre-coated plate. The plate was incubated for 2 hours at room temperature, washed, and incubated with 100 ul per well of S... View More
Product Details
SIL6
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Concentration
1 ng/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
The human IL-6 Standard is calibrated against NIBSC IL-6 Reference Standard lot (8)8/514. 1 Thermo pg = 1 NIBSC pg = 0.104 NIBSC units.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
1000 pg/ml when reconstituted at the recommend volume.
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
Target Information
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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