A closer look at urinary tract microbiome detection

Although widely used, urine culture-based methods for the detection of pathogens involved in urinary tract infections (UTIs) are cumbersome.  They require up to 30 hours of culturing time, lack sensitivity, are subjective, and have been shown to miss a significant percentage of positive cases.

In order to effectively determine disease etiology where pathogens are similar, analytically sensitive panels can be used to support laboratory research studies. Applied Biosystems Urinary Research Panels are real-time PCR syndromic panels designed to enable rapid analysis of a wide range of urinary tract microbiota in a scalable, low-cost per sample format in under five hours. While the advantages of molecular detection over culture-based methods have been known for some time, our solution is designed to deliver these benefits in an easy-to-implement, commercially viable format and can be tailored to your throughput needs.

Fast results—end-to-end workflow allows you to process samples and get answers in five hours

Analytically sensitive—detect slow-growing or difficult-to-culture microorganisms when traditional methods often lead to ambiguous results

Scalable—real-time PCR and our variety of assay formats enables you to run tens to hundreds of samples per day on a single instrument  

For Research Use Only. Not for use in diagnostic procedures.

Expand your urinary tract microbiome research with real-time PCR


Fast results

Our real-time PCR solution is optimized to deliver results in 5 hours—faster than traditional culture-based methods.  A complete, simple, and straightforward end-to-end solution is available to enable the processing of tens to hundreds of samples per day with minimal hands-on time.  

Detects slow-growing or difficult-to-culture uropathogens

Real-time PCR can detect slow-growing or difficult-to-culture microorganisms, and can be used when traditional microbiology techniques lead to ambiguous results. Utilizing real-time PCR techniques along with microorganism-specific TaqMan Assays enable rapid and accurate detection and categorization of multiple microorganisms involved in urinary tract microbiome composition and dynamics. Housing many of these TaqMan Assays on a single panel provides a molecular approach to researching the presence of multiple microorganisms and indicating the overall status of urinary tract microbiota. 

TaqMan OpenArray Urinary Tract Microbiota Comprehensive Plate

Targets available







Acinetobacter baumannii
Citrobacter freundii
Enterobacter aerogenes
Enterobacter cloacae                                           
Enterococcus faecalis                                           
Enterococcus faecium                                             
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Morganella morganii
Proteus mirabilis
Proteus vulgaris
Providencia stuartii
Pseudomonas aeruginosa
Staphylococcus saprophyticus
Streptococcus agalactiae
Candida albicans


Human RNase P







Scalable qPCR workflow

The QuantStudio Family of real-time PCR instruments can meet any throughput requirement. For higher throughput laboratories, leverage our OpenArray nanofluidic technology. This technology allows labs to process up to 600 urine samples per day on a single instrument.  A complete end-to-end solution is available and includes the Thermo Scientific KingFisher Flex benchtop automated DNA/RNA extraction instrument, convenient extraction and purification kits, and the OpenArray AccuFill System. Combined, these systems can automate sample prep and liquid handling to enable the processing of hundreds to thousands of samples per day.  


Whether you already own an instrument, or considering an expansion of your capabilities, your sample processing and throughput requirements can be addressed through the different formats compatible with your instrument (such as TaqMan array cards, 96- or 384-well plates, or single tube assays) with made-to-stock or custom panels.  Learn more about how OpenArray Technology can be an ideal solution for handling large numbers of research samples.

Explore QuantStudio PCR Systems, a flexible and scalable real-time PCR platform. Learn more ›

Ordering information

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.

© 2022 Thermo Fisher Scientific, Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license.

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