Electron Microscopy

Electron Microscopy for Pathology Research

Diagnostic electron microscopy for clinical disease analysis.

 

As global population and average longevity increase, there is inevitably a proportional rise in the number of patients in hospitals. For medical centers to keep pace, there is a need for fast, reliable screening of disease patterns. Transmission electron microscopy (TEM) is used as a phase-3 diagnostic solution to determine the nature of a group of specific diseases that can not be characterized with the standard/routine diagnostic methods such as molecular diagnostics and light microscopy.

As our knowledge of biological function at the nanoscale continues to grow, so does our understanding of pathological dysfunctions. TEMs have long played an important role in major pathology labs, providing a definitive diagnosis of certain conditions that are well beyond the resolving power of light microscopes. Examples include;

  • Membrane thickness variations in kidney disease
  • Mitochondrial changes in muscle disorders
  • Cilia in neonatal lung disease
  • Certain skin cancers and viral infections

In other cases, electron microscopy is used to complement the results of other diagnostic techniques. The use of TEM in medical diagnosis will surely increase as our knowledge of nanobiology continues to grow. 

Collaboration, automation, and accessibility

The direct and immediate human impact of diagnostic results makes accuracy and reliability paramount concerns, putting a high value on image quality. The ability to share digital images instantly, across your facility or across the world, is also important in the collaborative environment of modern medicine. The high quality of Thermo Fisher Scientific electron microscopes is coupled with a suite of tools for data management and sharing, ensuring results are available as soon as possible.  

Additionally, automated analysis of digital images is becoming increasingly common in medical diagnostics. Advances in automation and sample preparation promise to further improve the speed and reliability of TEM analysis, as well as greatly increase accessibility.

Lupus nephritis of kidney tissue imaged with TEM.
Lupus nephritis of kidney tissue imaged with transmission electron microscopy.
Renal biopsy of diseased kidney tissue imaged with TEM.
Renal biopsy of diseased kidney tissue imaged with transmission electron microscopy.
Detail of pancreatic endoplasmic reticulum imaged with TEM.
Detail of pancreatic endoplasmic reticulum imaged with transmission electron microscopy.

Techniques

Large Volume Analysis

A novel serial block-face imaging (SBFI) solution that combines multi-energy deconvolution scanning electron microscopy (MED-SEM) with in situ sectioning. Automation and ease-of-use functions provide isotropic resolution for large volume samples.

Learn more ›

Large Volume Analysis

A novel serial block-face imaging (SBFI) solution that combines multi-energy deconvolution scanning electron microscopy (MED-SEM) with in situ sectioning. Automation and ease-of-use functions provide isotropic resolution for large volume samples.

Learn more ›

Products

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Tundra Cryo-TEM

  • Structural information at biologically relevant resolution
  • Space efficient and cost effective
  • Easy, iterative sample optimization
  • Unique AI algorithms for streamlined data collection

Talos F200C TEM

  • Flexible EDS analysis reveals chemical information
  • High-contrast, high-quality TEM and STEM imaging
  • Ceta 16 Mpixel CMOS camera provides large field of view and high read-out speed

Talos L120C TEM

  • Increased stability
  • 4k × 4K Ceta CMOS camera
  • TEM magnification range from 25–650 kX
  • Flexible EDS analysis reveals chemical information

Prisma E SEM

  • Entry-level SEM with excellent image quality
  • Easy and quick sample loading and navigation for multiple samples
  • Compatible with a wide range of materials thanks to dedicated vacuum modes

Quattro ESEM

  • Ultra-versatile high-resolution FEG SEM with unique environmental capability (ESEM)
  • Observe all information from all samples with simultaneous SE and BSE imaging in every mode of operation

Apreo 2 SEM

  • High-performance SEM for all-round nanometer or sub-nanometer resolution
  • In-column T1 backscatter detector for sensitive, TV-rate materials contrast
  • Excellent performance at long working distance (10 mm)

Verios 5 XHR SEM

  • Monochromated SEM for sub-nanometer resolution over the full 1 keV to 30 keV energy range
  • Easy access to beam landing energies as low as 20 eV
  • Excellent stability with piezo stage as standard

VolumeScope 2 SEM

  • Isotropic 3D data from large volumes
  • High contrast and resolution in high and low vacuum modes
  • Simple switch between normal SEM use and serial block-face imaging

Helios Hydra DualBeam

  • 4 fast switchable ion species (Xe, Ar, O, N) for optimized PFIB processing of a widest range of materials
  • Ga-free TEM sample preparation
  • Extreme high resolution SEM imaging

Scios 2 DualBeam

  • Full support of magnetic and non-conductive samples
  • High throughput subsurface and 3D characterization
  • Advanced ease of use and automation capabilities

Maps Software

  • Acquire high resolution images over large areas
  • Easily find regions of interest
  • Automate image acquisition process
  • Correlate data from different sources

Auto Slice and View 4.0 Software

  • Automated serial sectioning for DualBeam
  • Multi-modal data acquisition (SEM, EDS, EBSD)
  • On-the-fly editing capabilities
  • Edge based cut placement

Inspect 3D Software

  • Image processing tools and filters for cross-correlation
  • Feature tracking for image alignment
  • Algebraic reconstruction technique for iterative projection comparison

Amira Software
Life Sciences

  • Import and process image data
  • Auto-segmentation, object separation, labeling
  • Analyze and quantify with automated statistics
  • Create images, animations and videos

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