Cryo-electron microscopy (cryo-EM) is a powerful technique for the fundamental study of viral function and infectivity as its visualization scale and resolution are ideally suited for analysis of the entire viral capsid.

One of the first 3D structures to be unraveled via cryo EM (single particle analysis) was a virus because their stable and symmetrical shape make them an ideal choice for cryo-EM method development, which benefits from structural symmetry. Thanks to cryo electron microscopy, clearer insights have been gained into the morphology and assembly strategy of viruses. In addition, cryo-EM plays a crucial role in the development of more efficient vaccines as it provides information on the specific binding properties of the antigen-antibody interface.

While single particle analysis was the method of choice for viral structural analysis, cellular cryo tomography has provided further insights into the viral replication, assembly, and maturation processes, granting an even broader perspective on viral-induced disease mechanisms.

Bacteriophage attached to cell wall; structure generated with virus EM (cryo electron tomography).
Structure of bacteriophage attached to salmonella cell wall, generated via cryo tomography (sub-tomogram averaging). 29.0 Å resolution. EMDB ID: 9010. Image generated with 3DBIONOTES.
Zika virus and antigen-antibody site generated with virus EM (3D single particle analysis).
3D single particle analysis reconstruction of the Zika virus and antigen-antibody target sites. Image courtesy of Sirohi et al., Purdue University
Human adenovirus 2 generated with virus EM (single particle analysis).
Human adenovirus 2 structure generated from single particle analysis data. 1.86 Å resolution. PDBe ID: 6E9D. Image generated with LiteMol.
Electron Microscopy

Virus Electron Microscopy

Immune electron microscopy for virology research.

Applications

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Drug Discovery

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.

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Infectious Disease Research

Cryo-EM techniques enable multiscale observations of 3D biological structures in their near-native states, informing faster, more efficient development of therapeutics.

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Structural Biology Research

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

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Pathology Research

Transmission electron microscopy (TEM) is used when the nature of the disease cannot be established via alternative methods. With nano-biological imaging, TEM provides accurate and reliable insight for certain pathologies.

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Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

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Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

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Single Particle Analysis

Single particle analysis (SPA) is a cryo electron microscopy technique that enables structural characterization at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.

Learn more ›

Cryo-Tomography

Cryo electron tomography (cryo-ET) delivers both structural information about individual proteins as well as their spatial arrangements within the cell. This makes it a truly unique technique and also explains why the method has such an enormous potential for cell biology. Cryo-ET can bridge the gap between light microscopy and near-atomic-resolution techniques like single-particle analysis.

Learn more ›

Products

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Glacios Cryo-TEM for
Life Sciences

  • Cryo-TEM
  • Small Footprint
  • Flexible Accelerating Voltage 80-200 kV
  • High Brightness X-FEG Electron Gun

Krios G4 Cryo-TEM for
Life Sciences

  • Improved ergonomics
  • Fits more easily into new and existing labs
  • Maximized productivity and automation
  • Best image quality for high-resolution 3D reconstruction

Talos Arctica TEM for
Life Sciences

  • Increased data acquisition speed
  • High data with robotic sample handling & automated loading
  • Unattended platform operation and automated data acquisition
  • Low cost of ownership with remote diagnostics and preventive service

Talos L120C for
Life Sciences

  • Increased stability
  • Multiple auto-functions
  • Quick sample exchange with robust vacuum system
  • High-quality cryo-imaging

Aquilos 2 Cryo-FIB for Life Sciences

  • Automation enables production of multiple lamellas
  • Target and extract your structure of interest with lift-out nano-manipulator
  • 3D visualization for high-resolution tomography

Vitrobot System

  • Fully Automated Sample vitrification
  • Blotting Device
  • Semi-Automated Grid Transfer
  • High Sample Throughput

EPU 2 Software

  • Microscope-embedded solution for single particle acquisition
  • Optimized for high-throughput particle collection
  • Compatible with film, CCD cameras, and direct electron detectors

Falcon 4 Detector

  • Leading detective quantum efficiency
  • 10x shorter exposure time than its predecessor
  • Fully embedded in Thermo Scientific software
  • Built in data management

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Electron microscopy services for
the life sciences

To ensure optimal system performance, we provide you access to a world-class network of field service experts, technical support, and certified spare parts.

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