Seamlessly scale up your analytical method for robust purification

With preparative columns, one size does not fit all. Whether you need unique chemistries for difficult separations to purify your compound of interest, robust columns for longer use, or budget-friendly solutions for routine applications, our preparative HPLC column portfolio has something to meet your needs. Our full range of customizable column hardware with optional XtendedLife technology offers robust, reproducible performance and expanded column capabilities in a wide variety of stationary phase chemistries, making it easy to scale up from your current analytical method.

  • Experience expanded column capabilities from our complete preparative HPLC column portfolio
  • Robust, customizable solutions to meet any application needs
  • Scale-up from your current analytical method with ease

Thermo Scientific preparative HPLC columns are available in 40+ stationary phase chemistries, allowing for maximum flexibility and ensuring that you have options to scale up your analytical workflows with chemistry continuity for easy method transfer.

Our preparative stationary phases are offered in three families:

Popular Thermo Scientific phasesWe have expanded our current offering of popular Thermo Scientific column chemistries, allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separation for a variety of applications. Available column chemistries include Hypersil GOLD, Accucore, MAbPac, and DNAPac.
Hypersil PREP phasesAn excellent choices for routine applications that require development from analytical to preparative HPLC scale. Available in C18, C8, silica, ODS, BDS, and phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide range of applications.
Hypersil PREP HS phasesOur PREP HS line features high surface area particles with a 100 Å pore size to maximize sample retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, silicone, and phenyl phases.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders. Please note that custom built columns are not eligible for returns.


Popular Thermo Scientific stationary phase details

 Pore size (Å)Surface area (m2/g)Carbon load (%)USP codepH range
Hypersil GOLD C1817522010L12 to 9
Hypersil GOLD C81752208L72 to 9
Hypersil GOLD aQ17522012L12 to 9
Hypersil GOLD C41752205L262 to 8
Hypersil GOLD Phenyl1752208L112 to 8
Hypercarb250120100L1090 to 14
Syncronis C1810032016L12 to 9
Syncronis HILIC1003205None2 to 8
Accucore XL C1880907L11 to 11
Accucore XL C880904L72 to 9
DNAPac RPProprietary wide pore22-24N/ANone0 to 14
DNAPac PA200NonporousN/AN/ANone4 to 10
MAbPacNonporousN/AN/ANone2 to 12
ProPacNonporousN/AN/ANone2 to 12


Hypersil PREP stationary phase details

 Pore size (Å)Surface area (m2/g)Carbon load (%)USP codepH range
Hypersil PREP C18 BDS14518511L12 to 8
Hypersil PREP C8 BDS1451857L72 to 8
Hypersil PREP ODS13019510L12 to 8
Hypersil PREP Si BDS1451850L32 to 7
Hypersil PREP Si1301950L32 to 7
Hypersil PREP Phenyl BDS 1451855L112 to 8


Hypersil PREP HS stationary phase details

 Pore size (Å)Surface area (m2/g)Carbon load (%)USP codepH range
Hypersil PREP C18 HS10035015L12 to 9
Hypersil PREP C8 HS1003507L72 to 8
Hypersil PREP Si HS1003500L32 to 7
Hypersil PREP Phenyl HS1003509L112 to 8


Preparative column hardware

We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:

Standard threaded and standard flange hardware

Standard threaded and standard flange hardware

  • Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
  • Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
  • Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
  • Standard hardware can be modified on request to be compatible with supercritical fluid chromatography.
XtendedLife hardware

XtendedLife hardware

  • Improves column lifetime by incorporating an internal Dynamic Axial Compression system, ensuring the media bed is continuously packed tightly.
  • Compatible with supercritical fluid chromatography and available in dimensions up to 50mm I.D.

Thermo Scientific Preparative Scale Liquid Chromatography Columns

Learn how Preparative HPLC columns help easily scale up a methods already developed on a small analytical column to a larger preparative HPLC column.


HPLC systems and consumables

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