Preparative HPLC Columns

Seamlessly scale up your analytical method for robust purification

For prep HPLC columns, one size does not fit all. Whether you need unique chemistries for difficult separations to purify your compound from impurities and side products, robust columns for longer use, or budget-friendly solutions for routine separations, our preparative column portfolio delivers. Our full range of column hardware with optional XtendedLife technology offers robust and reproducible performance along with a wide variety of chemistries makes it easy to scale up from your current analytical method.


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Scale up to more preparative HPLC column options

With columns in every format, media and size you will easily find a prep HPLC column for seamless method scale up.

  • Experience expanded column capabilities from our complete preparative HPLC column portfolio
  • Robust, customizable solutions to meet any application needs
  • Scale-up from your current analytical method with ease

If there is a preparative HPLC column you need that is not listed, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders. Please note that custom built columns are not eligible for returns.

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Preparative HPLC columns are available in 40+ chemistries allowing for maximum flexibility and options to scale up analytical methods with chemistry continuity for easy method transfer.

Our preparative stationary phases are offered in three families:

Hypersil PREP phases Excellent choices for routine applications which require development from analytical to preparative LC scale. Available in C18, C8, and Si BDS as well as ODS, Si and Phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide number of applications.
Hypersil PREP HS phases A high surface area particle with a 100Å pore size to maximize retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, Si and phenyl phases.
Premium Thermo Scientific phases We have expanded our current offering of premium Thermo Scientific column chemistries allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separations for a variety of applications.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

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Displayed are our most requested phases for preparative HPLC columns.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

Hypersil PREP Station Phase Details

  Pore Size (Å) Surface Area (m2/g) Carbon Load (%) USP Code pH Range
Hypersil PREP C18 BDS 145 185 11 L1 2 to 8
Hypersil PREP C8 BDS 145 185 7 L7 2 to 8
Hypersil PREP ODS 130 195 10 L1 2 to 8
Hypersil PREP Si BDS 145 185 0 L3 2 to 7
Hypersil PREP Si 130 195 0 L3 2 to 7
Hypersil PREP Phenyl BDS 145 185 5 L11 2 to 8

Hypersil PREP HS Station Phase Details

  Pore Size (Å) Surface Area (m2/g) Carbon Load (%) USP Code pH Range
Hypersil PREP C18 HS 100 350 15 L1 2 to 9
Hypersil PREP C8 HS 100 350 7 L7 2 to 8
Hypersil PREP Si HS 100 350 0 L3 2 to 7
Hypersil PREP Phenyl HS 100 350 9 L11 2 to 8

Premium Thermo Scientific Station Phase Details

  Pore Size (Å) Surface Area (m2/g) Carbon Load (%) USP Code pH Range
Hypersil GOLD C18 175 220 10 L1 2 to 9
Hypersil GOLD C8 175 220 8 L7 2 to 9
Hypersil GOLD aQ 175 220 12 L1 2 to 9
Hypersil GOLD C4 175 220 5 L26 2 to 8
Hypersil GOLD Phenyl 175 220 8 L11 2 to 8
Hypercarb 250 120 100 L109 0 to 14
Syncronis C18 100 320 16 L1 2 to 9
Syncronis HILIC 100 320 5 None 2 to 8
Accucore XL C18 80 90 7 L1 1 to 11
Accucore XL C8 80 90 4 L7 2 to 9
DNAPac RP Proprietary wide pore 22-24 N/A None 0 to 14
DNAPac PA200 Nonporous N/A N/A None 4 to 10
MAbPac Nonporous N/A N/A None 2 to 12
ProPac Nonporous N/A N/A None 2 to 12

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We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:

Standard Threaded and Standard Flange Hardware

Standard Threaded and Standard Flange Hardware

  • Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
  • Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
  • Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
  • Standard hardware can be modified on request to be compatible with SFC.
XtendedLife Hardware

XtendedLife hardware

  • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
  • Compatible with SFC and is available in dimensions up to 50mm i.d.

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Know what you need?

Know what you need?

Use the table at the back of the brochure to quickly locate your column part number so you can find it faster on Thermofisher.com.

Brochure

Top

Preparative HPLC columns are available in 40+ chemistries allowing for maximum flexibility and options to scale up analytical methods with chemistry continuity for easy method transfer.

Our preparative stationary phases are offered in three families:

Hypersil PREP phases Excellent choices for routine applications which require development from analytical to preparative LC scale. Available in C18, C8, and Si BDS as well as ODS, Si and Phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide number of applications.
Hypersil PREP HS phases A high surface area particle with a 100Å pore size to maximize retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, Si and phenyl phases.
Premium Thermo Scientific phases We have expanded our current offering of premium Thermo Scientific column chemistries allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separations for a variety of applications.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

Contact us

Top

Displayed are our most requested phases for preparative HPLC columns.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

Hypersil PREP Station Phase Details

  Pore Size (Å) Surface Area (m2/g) Carbon Load (%) USP Code pH Range
Hypersil PREP C18 BDS 145 185 11 L1 2 to 8
Hypersil PREP C8 BDS 145 185 7 L7 2 to 8
Hypersil PREP ODS 130 195 10 L1 2 to 8
Hypersil PREP Si BDS 145 185 0 L3 2 to 7
Hypersil PREP Si 130 195 0 L3 2 to 7
Hypersil PREP Phenyl BDS 145 185 5 L11 2 to 8

Hypersil PREP HS Station Phase Details

  Pore Size (Å) Surface Area (m2/g) Carbon Load (%) USP Code pH Range
Hypersil PREP C18 HS 100 350 15 L1 2 to 9
Hypersil PREP C8 HS 100 350 7 L7 2 to 8
Hypersil PREP Si HS 100 350 0 L3 2 to 7
Hypersil PREP Phenyl HS 100 350 9 L11 2 to 8

Premium Thermo Scientific Station Phase Details

  Pore Size (Å) Surface Area (m2/g) Carbon Load (%) USP Code pH Range
Hypersil GOLD C18 175 220 10 L1 2 to 9
Hypersil GOLD C8 175 220 8 L7 2 to 9
Hypersil GOLD aQ 175 220 12 L1 2 to 9
Hypersil GOLD C4 175 220 5 L26 2 to 8
Hypersil GOLD Phenyl 175 220 8 L11 2 to 8
Hypercarb 250 120 100 L109 0 to 14
Syncronis C18 100 320 16 L1 2 to 9
Syncronis HILIC 100 320 5 None 2 to 8
Accucore XL C18 80 90 7 L1 1 to 11
Accucore XL C8 80 90 4 L7 2 to 9
DNAPac RP Proprietary wide pore 22-24 N/A None 0 to 14
DNAPac PA200 Nonporous N/A N/A None 4 to 10
MAbPac Nonporous N/A N/A None 2 to 12
ProPac Nonporous N/A N/A None 2 to 12

Top

We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:

Standard Threaded and Standard Flange Hardware

Standard Threaded and Standard Flange Hardware

  • Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
  • Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
  • Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
  • Standard hardware can be modified on request to be compatible with SFC.
XtendedLife Hardware

XtendedLife hardware

  • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
  • Compatible with SFC and is available in dimensions up to 50mm i.d.

Top

Know what you need?

Know what you need?

Use the table at the back of the brochure to quickly locate your column part number so you can find it faster on Thermofisher.com.

Brochure

Top

Hypersil PREP Columns

Hypersil PREP HS Columns

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

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Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

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Hypersil PREP Columns

Hypersil PREP HS Columns

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Top

Hypersil PREP Columns

Hypersil PREP HS Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Top

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Top

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Top

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Top

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Top

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Top

Our Hypersil Chiral columns allow you to separate a majority of enantiomers. To maximize screening efficiency, these columns can be ordered in kits and all columns are available up to preparative scale.

Hypersil Chiral JT HPLC Columns Hypersil Chiral AT HPLC Columns Hypersil Chiral OT HPLC Columns
  • Chemistry: Tris-(4-methylbenzoyl)-cellulose modified silica
  • Best starting point for method development
  • Chemistry: Amylose tris-3,5-dimethylphenylcarbamate modified silica
  • Highest generality of all chiral phases
  • No aromatic ring necessary
  • No inversion of elution order possible
  • Chemistry: Cellulose tris-(3.5-dimethylphenyl-carbamate) modified silica
  • Highly versatile chiral phase.
  • No aromatic ring necessary.
  • No inversion of elution order possible

There are many other Chiral Stationary Phases (CSP’S) available from Thermo Scientific including immobilized brush-type phases. Contact your local sales representative with your specific requirements for chiral separations.

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Ready to scale up your analytical method?

Ready to scale up your analytical method?

Use this online tool to determine best starting conditions for scaling methods between analytical to preparative HPLC conditions. Just insert your current method conditions and your new column dimensions to get up and running faster.

Calculate your starting conditions


What is Prep LC?

Preparative scale liquid chromatography is used for the purification and isolation of molecules which are of value in the pharmaceutical, biopharma and associated chemical industries. The production of highly pure compounds in these industries is increasingly important in terms of the chemical activity, toxicity and usefulness for screening in highly defined and often costly experimental processes.

Often these processes start on an analytical scale with only a few micrograms of material e.g. for enzymes in biopharmaceutical applications, or a few milligrams e.g. for structural or identification of synthesized or natural products. Further along the process, gram quantities are usually required for standard and reference compounds or compounds targeted for pharmacology and toxicology testing procedures. In pharmaceutical environments, as a drug target compound moves further in the drug development pipeline, there is a need to “scale up” the reaction to create more volume of the targeted compound.

Thermo Fisher Scientific provides scientists a full portfolio of analytical and preparative HPLC columns to allow for seamless and robust method development and scale up using similar media for continuity. With a wide variety of stationary phases and a wide selection of column hardware and dimensions, Thermo Fisher Scientific can meet any application needs.

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