Preparative HPLC Columns

Seamlessly scale up your analytical method for robust purification

For prep HPLC columns, one size does not fit all. Whether you need unique chemistries for difficult separations to purify your compound from impurities and side products, robust columns for longer use, or budget-friendly solutions for routine separations, our preparative column portfolio delivers. Our full range of column hardware with optional XtendedLife technology offers robust and reproducible performance along with a wide variety of chemistries makes it easy to scale up from your current analytical method.


On this page

Scale up to more preparative HPLC column options

With columns in every format, media and size you will easily find a prep HPLC column for seamless method scale up.

  • Experience expanded column capabilities from our complete preparative HPLC column portfolio
  • Robust, customizable solutions to meet any application needs
  • Scale-up from your current analytical method with ease

If there is a preparative HPLC column you need that is not listed, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders. Please note that custom built columns are not eligible for returns.

Contact us

Preparative HPLC columns are available in 40+ chemistries allowing for maximum flexibility and options to scale up analytical methods with chemistry continuity for easy method transfer.

Our preparative stationary phases are offered in three families:

Hypersil PREP phasesExcellent choices for routine applications which require development from analytical to preparative LC scale. Available in C18, C8, and Si BDS as well as ODS, Si and Phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide number of applications.
Hypersil PREP HS phasesA high surface area particle with a 100Å pore size to maximize retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, Si and phenyl phases.
Premium Thermo Scientific phasesWe have expanded our current offering of premium Thermo Scientific column chemistries allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separations for a variety of applications.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

Contact us

Displayed are our most requested phases for preparative HPLC columns.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

Hypersil PREP Stationary Phase Details

 Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil PREP C18 BDS14518511L12 to 8
Hypersil PREP C8 BDS1451857L72 to 8
Hypersil PREP ODS13019510L12 to 8
Hypersil PREP Si BDS1451850L32 to 7
Hypersil PREP Si1301950L32 to 7
Hypersil PREP Phenyl BDS 1451855L112 to 8

Hypersil PREP HS Stationary Phase Details

 Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil PREP C18 HS10035015L12 to 9
Hypersil PREP C8 HS1003507L72 to 8
Hypersil PREP Si HS1003500L32 to 7
Hypersil PREP Phenyl HS1003509L112 to 8

Premium Thermo Scientific Stationary Phase Details

 Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil GOLD C1817522010L12 to 9
Hypersil GOLD C81752208L72 to 9
Hypersil GOLD aQ17522012L12 to 9
Hypersil GOLD C41752205L262 to 8
Hypersil GOLD Phenyl1752208L112 to 8
Hypercarb250120100L1090 to 14
Syncronis C1810032016L12 to 9
Syncronis HILIC1003205None2 to 8
Accucore XL C1880907L11 to 11
Accucore XL C880904L72 to 9
DNAPac RPProprietary wide pore22-24N/ANone0 to 14
DNAPac PA200NonporousN/AN/ANone4 to 10
MAbPacNonporousN/AN/ANone2 to 12
ProPacNonporousN/AN/ANone2 to 12

Pack columns yourself?

10 µm and 7 µm particle size media are available in bulk in a range of stationary phase chemistries.

Hypersil PREP HS

 Particle Size (µm)Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil PREP C18 HS1010035015L12 to 9
Hypersil PREP C8 HS101003507L72 to 8
Hypersil PREP Si HS101003500L32 to 7
Hypersil PREP Phenyl HS101003509L112 to 8
xtendedlife-column-hardware-270x195

Want best performance time after time?

Repacking services are now available for all preparative columns with XL dimensions in our current portfolio, meaning no ongoing hardware costs. Simply return your used column to the Thermo Fisher Scientific to be repacked.

Find the repacking service that meets your needs below:

We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:

Standard Threaded and Standard Flange Hardware

Standard Threaded and Standard Flange Hardware

  • Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
  • Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
  • Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
  • Standard hardware can be modified on request to be compatible with SFC.
XtendedLife Hardware

XtendedLife hardware

  • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
  • Compatible with SFC and is available in dimensions up to 50mm i.d.

Preparative HPLC columns are available in 40+ chemistries allowing for maximum flexibility and options to scale up analytical methods with chemistry continuity for easy method transfer.

Our preparative stationary phases are offered in three families:

Hypersil PREP phasesExcellent choices for routine applications which require development from analytical to preparative LC scale. Available in C18, C8, and Si BDS as well as ODS, Si and Phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide number of applications.
Hypersil PREP HS phasesA high surface area particle with a 100Å pore size to maximize retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, Si and phenyl phases.
Premium Thermo Scientific phasesWe have expanded our current offering of premium Thermo Scientific column chemistries allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separations for a variety of applications.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

Contact us

Displayed are our most requested phases for preparative HPLC columns.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.

Hypersil PREP Stationary Phase Details

 Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil PREP C18 BDS14518511L12 to 8
Hypersil PREP C8 BDS1451857L72 to 8
Hypersil PREP ODS13019510L12 to 8
Hypersil PREP Si BDS1451850L32 to 7
Hypersil PREP Si1301950L32 to 7
Hypersil PREP Phenyl BDS 1451855L112 to 8

Hypersil PREP HS Stationary Phase Details

 Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil PREP C18 HS10035015L12 to 9
Hypersil PREP C8 HS1003507L72 to 8
Hypersil PREP Si HS1003500L32 to 7
Hypersil PREP Phenyl HS1003509L112 to 8

Premium Thermo Scientific Stationary Phase Details

 Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil GOLD C1817522010L12 to 9
Hypersil GOLD C81752208L72 to 9
Hypersil GOLD aQ17522012L12 to 9
Hypersil GOLD C41752205L262 to 8
Hypersil GOLD Phenyl1752208L112 to 8
Hypercarb250120100L1090 to 14
Syncronis C1810032016L12 to 9
Syncronis HILIC1003205None2 to 8
Accucore XL C1880907L11 to 11
Accucore XL C880904L72 to 9
DNAPac RPProprietary wide pore22-24N/ANone0 to 14
DNAPac PA200NonporousN/AN/ANone4 to 10
MAbPacNonporousN/AN/ANone2 to 12
ProPacNonporousN/AN/ANone2 to 12

Pack columns yourself?

10 µm and 7 µm particle size media are available in bulk in a range of stationary phase chemistries.

Hypersil PREP HS

 Particle Size (µm)Pore Size (Å)Surface Area (m2/g)Carbon Load (%)USP CodepH Range
Hypersil PREP C18 HS1010035015L12 to 9
Hypersil PREP C8 HS101003507L72 to 8
Hypersil PREP Si HS101003500L32 to 7
Hypersil PREP Phenyl HS101003509L112 to 8
xtendedlife-column-hardware-270x195

Want best performance time after time?

Repacking services are now available for all preparative columns with XL dimensions in our current portfolio, meaning no ongoing hardware costs. Simply return your used column to the Thermo Fisher Scientific to be repacked.

Find the repacking service that meets your needs below:

We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:

Standard Threaded and Standard Flange Hardware

Standard Threaded and Standard Flange Hardware

  • Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
  • Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
  • Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
  • Standard hardware can be modified on request to be compatible with SFC.
XtendedLife Hardware

XtendedLife hardware

  • Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
  • Compatible with SFC and is available in dimensions up to 50mm i.d.

Hypersil PREP Columns

Hypersil PREP HS Columns

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Hypersil PREP Columns

Hypersil PREP HS Columns

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Hypersil PREP Columns

Hypersil PREP HS Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Premium Thermo Scientific Columns

Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.

Our Hypersil Chiral columns allow you to separate a majority of enantiomers. To maximize screening efficiency, these columns can be ordered in kits and all columns are available up to preparative scale.

Hypersil Chiral JT HPLC ColumnsHypersil Chiral AT HPLC ColumnsHypersil Chiral OT HPLC Columns
  • Chemistry: Tris-(4-methylbenzoyl)-cellulose modified silica
  • Best starting point for method development
  • Chemistry: Amylose tris-3,5-dimethylphenylcarbamate modified silica
  • Highest generality of all chiral phases
  • No aromatic ring necessary
  • No inversion of elution order possible
  • Chemistry: Cellulose tris-(3.5-dimethylphenyl-carbamate) modified silica
  • Highly versatile chiral phase.
  • No aromatic ring necessary.
  • No inversion of elution order possible

There are many other Chiral Stationary Phases (CSP’S) available from Thermo Scientific including immobilized brush-type phases. Contact your local sales representative with your specific requirements for chiral separations.


Ready to scale up your analytical method?

Ready to scale up your analytical method?

Use this online tool to determine best starting conditions for scaling methods between analytical to preparative HPLC conditions. Just insert your current method conditions and your new column dimensions to get up and running faster.

Calculate your starting conditions


What is Prep LC?

Preparative scale liquid chromatography is used for the purification and isolation of molecules which are of value in the pharmaceutical, biopharma and associated chemical industries. The production of highly pure compounds in these industries is increasingly important in terms of the chemical activity, toxicity and usefulness for screening in highly defined and often costly experimental processes.

Often these processes start on an analytical scale with only a few micrograms of material e.g. for enzymes in biopharmaceutical applications, or a few milligrams e.g. for structural or identification of synthesized or natural products. Further along the process, gram quantities are usually required for standard and reference compounds or compounds targeted for pharmacology and toxicology testing procedures. In pharmaceutical environments, as a drug target compound moves further in the drug development pipeline, there is a need to “scale up” the reaction to create more volume of the targeted compound.

Thermo Fisher Scientific provides scientists a full portfolio of analytical and preparative HPLC columns to allow for seamless and robust method development and scale up using similar media for continuity. With a wide variety of stationary phases and a wide selection of column hardware and dimensions, Thermo Fisher Scientific can meet any application needs.

Learn more



On-demand Consumables for chromatography webinar series: Learn at your own pace on a wide range of topics from column troubleshooting, method transfer, to tips for successful low-flow and more​

Watch now

CMD SchemaApp code