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Preparative HPLC Columns
Seamlessly scale up your analytical method for robust purification
For prep HPLC columns, one size does not fit all. Whether you need unique chemistries for difficult separations to purify your compound from impurities and side products, robust columns for longer use, or budget-friendly solutions for routine separations, our preparative column portfolio delivers. Our full range of column hardware with optional XtendedLife technology offers robust and reproducible performance along with a wide variety of chemistries makes it easy to scale up from your current analytical method.
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Scale up to more preparative HPLC column options
With columns in every format, media and size you will easily find a prep HPLC column for seamless method scale up.
- Experience expanded column capabilities from our complete preparative HPLC column portfolio
- Robust, customizable solutions to meet any application needs
- Scale-up from your current analytical method with ease
If there is a preparative HPLC column you need that is not listed, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders. Please note that custom built columns are not eligible for returns.
Preparative HPLC columns are available in 40+ chemistries allowing for maximum flexibility and options to scale up analytical methods with chemistry continuity for easy method transfer.
Our preparative stationary phases are offered in three families:
| Hypersil PREP phases | Excellent choices for routine applications which require development from analytical to preparative LC scale. Available in C18, C8, and Si BDS as well as ODS, Si and Phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide number of applications. |
| Hypersil PREP HS phases | A high surface area particle with a 100Å pore size to maximize retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, Si and phenyl phases. |
| Premium Thermo Scientific phases | We have expanded our current offering of premium Thermo Scientific column chemistries allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separations for a variety of applications. |
If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.
Displayed are our most requested phases for preparative HPLC columns.
If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.
Hypersil PREP Stationary Phase Details
| Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|
| Hypersil PREP C18 BDS | 145 | 185 | 11 | L1 | 2 to 8 |
| Hypersil PREP C8 BDS | 145 | 185 | 7 | L7 | 2 to 8 |
| Hypersil PREP ODS | 130 | 195 | 10 | L1 | 2 to 8 |
| Hypersil PREP Si BDS | 145 | 185 | 0 | L3 | 2 to 7 |
| Hypersil PREP Si | 130 | 195 | 0 | L3 | 2 to 7 |
| Hypersil PREP Phenyl BDS | 145 | 185 | 5 | L11 | 2 to 8 |
Hypersil PREP HS Stationary Phase Details
| Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|
| Hypersil PREP C18 HS | 100 | 350 | 15 | L1 | 2 to 9 |
| Hypersil PREP C8 HS | 100 | 350 | 7 | L7 | 2 to 8 |
| Hypersil PREP Si HS | 100 | 350 | 0 | L3 | 2 to 7 |
| Hypersil PREP Phenyl HS | 100 | 350 | 9 | L11 | 2 to 8 |
Premium Thermo Scientific Stationary Phase Details
| Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|
| Hypersil GOLD C18 | 175 | 220 | 10 | L1 | 2 to 9 |
| Hypersil GOLD C8 | 175 | 220 | 8 | L7 | 2 to 9 |
| Hypersil GOLD aQ | 175 | 220 | 12 | L1 | 2 to 9 |
| Hypersil GOLD C4 | 175 | 220 | 5 | L26 | 2 to 8 |
| Hypersil GOLD Phenyl | 175 | 220 | 8 | L11 | 2 to 8 |
| Hypercarb | 250 | 120 | 100 | L109 | 0 to 14 |
| Syncronis C18 | 100 | 320 | 16 | L1 | 2 to 9 |
| Syncronis HILIC | 100 | 320 | 5 | None | 2 to 8 |
| Accucore XL C18 | 80 | 90 | 7 | L1 | 1 to 11 |
| Accucore XL C8 | 80 | 90 | 4 | L7 | 2 to 9 |
| DNAPac RP | Proprietary wide pore | 22-24 | N/A | None | 0 to 14 |
| DNAPac PA200 | Nonporous | N/A | N/A | None | 4 to 10 |
| MAbPac | Nonporous | N/A | N/A | None | 2 to 12 |
| ProPac | Nonporous | N/A | N/A | None | 2 to 12 |
Pack columns yourself?
10 µm and 7 µm particle size media are available in bulk in a range of stationary phase chemistries.
Hypersil PREP HS
| Particle Size (µm) | Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|---|
| Hypersil PREP C18 HS | 10 | 100 | 350 | 15 | L1 | 2 to 9 |
| Hypersil PREP C8 HS | 10 | 100 | 350 | 7 | L7 | 2 to 8 |
| Hypersil PREP Si HS | 10 | 100 | 350 | 0 | L3 | 2 to 7 |
| Hypersil PREP Phenyl HS | 10 | 100 | 350 | 9 | L11 | 2 to 8 |
Want best performance time after time?
Repacking services are now available for all preparative columns with XL dimensions in our current portfolio, meaning no ongoing hardware costs. Simply return your used column to the Thermo Fisher Scientific to be repacked.
Find the repacking service that meets your needs below:
- Hypersil PREP BDS C18 HPLC Columns
- Hypersil PREP BDS C8 HPLC Columns
- Hypersil PREP BDS Phenyl HPLC Columns
- Hypersil PREP BDS Si HPLC Columns
- Hypersil GOLD PREP aQ HPLC Columns
- Hypersil GOLD PREP C4 HPLC Columns
- Hypersil GOLD PREP C8 HPLC Columns
- Hypersil GOLD PREP C18 HPLC Columns
- Hypersil PREP ODS C18 HPLC Columns
We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:
Standard Threaded and Standard Flange Hardware
- Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
- Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
- Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
- Standard hardware can be modified on request to be compatible with SFC.
XtendedLife hardware
- Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
- Compatible with SFC and is available in dimensions up to 50mm i.d.
Preparative HPLC columns are available in 40+ chemistries allowing for maximum flexibility and options to scale up analytical methods with chemistry continuity for easy method transfer.
Our preparative stationary phases are offered in three families:
| Hypersil PREP phases | Excellent choices for routine applications which require development from analytical to preparative LC scale. Available in C18, C8, and Si BDS as well as ODS, Si and Phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide number of applications. |
| Hypersil PREP HS phases | A high surface area particle with a 100Å pore size to maximize retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, Si and phenyl phases. |
| Premium Thermo Scientific phases | We have expanded our current offering of premium Thermo Scientific column chemistries allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separations for a variety of applications. |
If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.
Displayed are our most requested phases for preparative HPLC columns.
If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders.
Hypersil PREP Stationary Phase Details
| Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|
| Hypersil PREP C18 BDS | 145 | 185 | 11 | L1 | 2 to 8 |
| Hypersil PREP C8 BDS | 145 | 185 | 7 | L7 | 2 to 8 |
| Hypersil PREP ODS | 130 | 195 | 10 | L1 | 2 to 8 |
| Hypersil PREP Si BDS | 145 | 185 | 0 | L3 | 2 to 7 |
| Hypersil PREP Si | 130 | 195 | 0 | L3 | 2 to 7 |
| Hypersil PREP Phenyl BDS | 145 | 185 | 5 | L11 | 2 to 8 |
Hypersil PREP HS Stationary Phase Details
| Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|
| Hypersil PREP C18 HS | 100 | 350 | 15 | L1 | 2 to 9 |
| Hypersil PREP C8 HS | 100 | 350 | 7 | L7 | 2 to 8 |
| Hypersil PREP Si HS | 100 | 350 | 0 | L3 | 2 to 7 |
| Hypersil PREP Phenyl HS | 100 | 350 | 9 | L11 | 2 to 8 |
Premium Thermo Scientific Stationary Phase Details
| Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|
| Hypersil GOLD C18 | 175 | 220 | 10 | L1 | 2 to 9 |
| Hypersil GOLD C8 | 175 | 220 | 8 | L7 | 2 to 9 |
| Hypersil GOLD aQ | 175 | 220 | 12 | L1 | 2 to 9 |
| Hypersil GOLD C4 | 175 | 220 | 5 | L26 | 2 to 8 |
| Hypersil GOLD Phenyl | 175 | 220 | 8 | L11 | 2 to 8 |
| Hypercarb | 250 | 120 | 100 | L109 | 0 to 14 |
| Syncronis C18 | 100 | 320 | 16 | L1 | 2 to 9 |
| Syncronis HILIC | 100 | 320 | 5 | None | 2 to 8 |
| Accucore XL C18 | 80 | 90 | 7 | L1 | 1 to 11 |
| Accucore XL C8 | 80 | 90 | 4 | L7 | 2 to 9 |
| DNAPac RP | Proprietary wide pore | 22-24 | N/A | None | 0 to 14 |
| DNAPac PA200 | Nonporous | N/A | N/A | None | 4 to 10 |
| MAbPac | Nonporous | N/A | N/A | None | 2 to 12 |
| ProPac | Nonporous | N/A | N/A | None | 2 to 12 |
Pack columns yourself?
10 µm and 7 µm particle size media are available in bulk in a range of stationary phase chemistries.
Hypersil PREP HS
| Particle Size (µm) | Pore Size (Å) | Surface Area (m2/g) | Carbon Load (%) | USP Code | pH Range | |
|---|---|---|---|---|---|---|
| Hypersil PREP C18 HS | 10 | 100 | 350 | 15 | L1 | 2 to 9 |
| Hypersil PREP C8 HS | 10 | 100 | 350 | 7 | L7 | 2 to 8 |
| Hypersil PREP Si HS | 10 | 100 | 350 | 0 | L3 | 2 to 7 |
| Hypersil PREP Phenyl HS | 10 | 100 | 350 | 9 | L11 | 2 to 8 |
Want best performance time after time?
Repacking services are now available for all preparative columns with XL dimensions in our current portfolio, meaning no ongoing hardware costs. Simply return your used column to the Thermo Fisher Scientific to be repacked.
Find the repacking service that meets your needs below:
- Hypersil PREP BDS C18 HPLC Columns
- Hypersil PREP BDS C8 HPLC Columns
- Hypersil PREP BDS Phenyl HPLC Columns
- Hypersil PREP BDS Si HPLC Columns
- Hypersil GOLD PREP aQ HPLC Columns
- Hypersil GOLD PREP C4 HPLC Columns
- Hypersil GOLD PREP C8 HPLC Columns
- Hypersil GOLD PREP C18 HPLC Columns
- Hypersil PREP ODS C18 HPLC Columns
We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:
Standard Threaded and Standard Flange Hardware
- Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
- Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
- Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
- Standard hardware can be modified on request to be compatible with SFC.
XtendedLife hardware
- Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
- Compatible with SFC and is available in dimensions up to 50mm i.d.
Hypersil PREP Columns
Hypersil PREP HS Columns
Premium Thermo Scientific Columns
- Hypersil GOLD C18 HPLC Columns
- Hypersil GOLD aQ HPLC Columns
- Syncronis C18 HPLC Columns
- Acclaim 120 C18 HPLC Columns
- Hypersil BDS C18 HPLC Columns
- Hypersil ODS C18 HPLC Columns
- BETASIL C18 HPLC Columns
- BioBasic 18 HPLC Columns
- HyPURITY Aquastar HPLC Columns
- Accucore XL C18 HPLC Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Hypersil PREP Columns
Hypersil PREP HS Columns
Premium Thermo Scientific Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Premium Thermo Scientific Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Hypersil PREP Columns
Hypersil PREP HS Columns
Premium Thermo Scientific Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Hypersil PREP Columns
Hypersil PREP HS Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Premium Thermo Scientific Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Premium Thermo Scientific Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Premium Thermo Scientific Columns
- DNAPac PA Oligonucleotide HPLC Columns
- MAbPac SCX-10 HPLC Columns
- ProPac SAX-10 HPLC Columns
- ProPac SCX-10 HPLC Columns
- ProPac WAX-10 HPLC Columns
- ProPac WCX-10 HPLC Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Premium Thermo Scientific Columns
- MAbPac HIC-10 HPLC Columns
- MAbPac HIC-20 HPLC Columns
- MAbPac HIC-Butyl HPLC Columns
- ProPac HIC-10 HPLC Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Premium Thermo Scientific Columns
- Hypercarb HPLC Columns
- Acclaim Polar Advantage II HPLC Columns
- Hypersil APS-2 HPLC Columns
- DNAPac RP HPLC Columns
Guard columns are available, and can be found within their respective phase type. Our guard columns require guard column holders.
Our Hypersil Chiral columns allow you to separate a majority of enantiomers. To maximize screening efficiency, these columns can be ordered in kits and all columns are available up to preparative scale.
| Hypersil Chiral JT HPLC Columns | Hypersil Chiral AT HPLC Columns | Hypersil Chiral OT HPLC Columns |
|---|---|---|
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There are many other Chiral Stationary Phases (CSP’S) available from Thermo Scientific including immobilized brush-type phases. Contact your local sales representative with your specific requirements for chiral separations.
Chromatography Consumables Resources and Information
Find interactive tools and calculators, guides to select the right products, equivalent product finders, and get in contact with our consumables technical support team to answer your application questions.
Ready to scale up your analytical method?
Use this online tool to determine best starting conditions for scaling methods between analytical to preparative HPLC conditions. Just insert your current method conditions and your new column dimensions to get up and running faster.
What is Prep LC?
Preparative scale liquid chromatography is used for the purification and isolation of molecules which are of value in the pharmaceutical, biopharma and associated chemical industries. The production of highly pure compounds in these industries is increasingly important in terms of the chemical activity, toxicity and usefulness for screening in highly defined and often costly experimental processes.
Often these processes start on an analytical scale with only a few micrograms of material e.g. for enzymes in biopharmaceutical applications, or a few milligrams e.g. for structural or identification of synthesized or natural products. Further along the process, gram quantities are usually required for standard and reference compounds or compounds targeted for pharmacology and toxicology testing procedures. In pharmaceutical environments, as a drug target compound moves further in the drug development pipeline, there is a need to “scale up” the reaction to create more volume of the targeted compound.
Thermo Fisher Scientific provides scientists a full portfolio of analytical and preparative HPLC columns to allow for seamless and robust method development and scale up using similar media for continuity. With a wide variety of stationary phases and a wide selection of column hardware and dimensions, Thermo Fisher Scientific can meet any application needs.
2022/2023 Chromatography Sample Preparation Catalog
Access the latest interactive version of our Chromatography Consumables & Columns Catalog. Our enhanced online version features easier access to product information, key application notes and brand-new selector tools.
Discover live and on-demand webinars that enable you to improve productivity, maintain compliance, and learn about the constantly evolving scientific landscape.
Resources
Style Sheet for Global Design System
Style Sheet for Komodo Tabs
CMD SchemaApp code