Quantifiler® Duo Kit Configuration
| Target | Marker | Size | Dye |
|---|---|---|---|
| Human DNA | RPPH1 (Ribonuclease P RNA component H1) | 140 bp | VIC® |
| Human Male DNA | SRY (Sex determining Region Y) | 130 bp | FAM™ |
| Internal PCR Control (IPC) | Artificial Template | 130 bp | NED™ |
- Increased amplicon sizes for improved prediction of STR amplification performance and estimation of DNA quantity in degraded samples
- Similar amplicon sizes for all three targets to avoid preferential PCR
- Single copy targets for superior accuracy and consistency in quantification results (multi-copy targets are affected by variability between individuals and decrease correlation with the single copy targets in STR kits)
- Validated on the 7500 Real-Time PCR System using SDS v1.2.3 software