Target Marker Size Dye
Human DNA RPPH1 (Ribonuclease P RNA component H1) 140 bp VIC®
Human Male DNA SRY (Sex determining Region Y) 130 bp FAM™
Internal PCR Control (IPC) Artificial Template 130 bp NED™

  • Increased amplicon sizes for improved prediction of STR amplification performance and estimation of DNA quantity in degraded samples
  • Similar amplicon sizes for all three targets to avoid preferential PCR
  • Single copy targets for superior accuracy and consistency in quantification results (multi-copy targets are affected by variability between individuals and decrease correlation with the single copy targets in STR kits)
  • Validated on the 7500 Real-Time PCR System using SDS v1.2.3 software