ISO 18385

Forensic DNA analysis has been used since 1985 when minisatellites and Southern blotting were the technology of the day. In the early 1990s, short tandem repeats (STRs) were introduced as a new polymorphic DNA marker type and have since become the gold standard for human identification in DNA databases, criminal casework, paternity and kinship analyses, and identification of missing persons. Recent advances in designing large multiplex STR panels have greatly increased the power of discrimination to aid in inclusion and exclusion decisions in human identification applications. Regional requirements for specific markers vary but with ongoing international data sharing efforts, STR PCR amplification kits need maximum commonality in markers to make more cross-border identifications. Our PCR amplification kits are manufactured at our location in Warrington, UK—a facility that meets the guidelines for ISO 18385 certification.

Which autosomal STR kit is right for your lab?

 

Locus

GlobalFiler kits

VeriFiler kits

NGM kits

D13S317XX 
D7S820XX 
CSF1POXX 
D5S818XX 
TPOXXX 
D16S539XXX
AMELXXX
D19S433XXX
D2S1338XXX
D18S51XXX
FGAXXX
D21S11XXX
D8S1179XXX
VWAXXX
TH01XXX
D3S1358XXX
D1S1656XXX
D12S391XXX
D2S441XXX
D10S1248XXX
D22S1045XXX
SE33X X
DYS391X  
YindelXXNGM Detect kit only
Penta D X 
Penta E X 
D6S1043 X 
IQCGlobalFiler IQC kit onlyVeriFiler Plus kit onlyNGM Detect kit only


PCR amplification products

GlobalFiler STR Kits combine reduced amplification time with maximum data recovery power. The 6-dye, 24-locus technology is designed to deliver superior lab performance. Both the GlobalFiler and GlobalFiler Express kits are approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

VeriFiler STR Kits offer superior genotyping results from single-source and challenging casework samples using a 25-marker STR multiplex assay that includes two gender determination markers, as well as the highly discriminatory Penta D and Penta E markers. VeriFiler Plus and VeriFiler Express kits are approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

The Applied Biosystems NGM family of kits delivers exceptional data quality and robustness along with powerful discrimination to support European cross-border data sharing initiatives. The NGM kits amplify all Applied Biosystems SGM Plus kit loci together with additional loci in the expanded European Standard Set as recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) (SE33, D2S1338, D3S1358, D8S1179, D16S539, D18S51, D19S433, D21S11, FGA, TH01, vWA, D10S1248, D22S1045, D2S441, D1S1656, D12S391 and amelogenin).

Y-STR analysis focuses on STR markers found on the Y chromosome, only carried by male individuals. For forensic labs, the most common application of Y-STRs is in criminal cases such as sexual assault, where evidence samples are difficult to resolve with traditional autosomal STR analysis due to low amounts of male DNA present in a high background of female DNA. The Yfiler Plus kit is approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

Optimized for casework, the AmpFℓSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence.

Developed with input from forensic DNA laboratories, Applied Biosystems GeneMapper ID-X Software helps save time and increases workflow efficiency. When combined with the Applied Biosystems  Data Collection Software and Applied Biosystems STR kits, GeneMapper ID-X helps significantly enhance productivity and confidence, as well as IT support and compliance, in your HID laboratory’s data interpretation workflow.

Forensic DNA analysis systems

seqstudio-genetic-analyzer-v2

The Applied Biosystems SeqStudio Genetic Analyzer for HID is a 4-capillary, easy-to-use, and convenient benchtop system that delivers gold-standard STR fragment analysis and Sanger sequencing without having to change polymer type or array. It is easily used across a broad range of forensic STR kits and applications as well as by multiple users. 

3500-dx-cs2-r4-v2

The Applied Biosystems 3500 Series instruments were the first genetic analyzers designed with a specific feature set and workflow for human identification (HID) applications. The complete system combines the instrument with Applied Biosystems reagents, consumables, and software, as well as industry-leading support—providing an integrated HID solution that significantly improves ease of use and application efficiency.

VeritiPro Thermal Cycler for Human Identification

The Applied Biosystems VeritiPro Thermal Cycler for HID delivers high-end performance and proven reliability with advanced temperature control technology. It is validated according to the Scientific Working Group on DNA Analysis Methods (SWGDAM) with Applied Biosystems STR PCR amplification kits and Precision ID NGS panels.

SF-proflex-v3

The ProFlex PCR System combines the reliability and performance of Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Multiple block configurations are available that allow you to maximize your throughput.

Featured videos

Flexibility of 6-dye Chemistries in recovering maximum information

Dr. Antonio Alonso, a DNA expert at the National Institute of Toxicology and Forensic Sciences, Madrid (Spain), discusses the need for forensic laboratories to make the transition to 6-dye Chemistry technology in order to gain workflow efficiency and reduce operating costs. He highlights the value of Y-STR analysis in resolving sexual assault cases and provides the example of an 18-year-old rape/murder cold case in Spain that was solved using Applied Biosystems Yfiler Plus chemistry.

Value of Y-STRs in resolving mixture samples

Y-STR analysis can increase the success rate of investigations into sexual assaults, particularly in cases where only non-sperm traces may be available such as saliva, skin, finger nails, contact & touch traces. Such mixture samples contain small amounts of male DNA in a high background of female DNA and autosomal testing often does not yield a complete profile. Dr. Franz Neuhuber, Head of Forensic Molecular Biology Department at University of Salzburg, Austria shares details of an attempted rape case that prompted the lab to adopt Y-STR analysis.

Optimizing the sexual assault workflow: Applied Biosystems Quantifiler Trio, Yfiler Plus and Identifiler kits

Donna Foskin, senior scientist at the Institute of Environmental Science and Research (ESR), describes how they process sexual assault kits. To start, the details of the case are taken into consideration along with the length of time that has passed since the assault occurred. Once the DNA has been extracted, the team uses Applied Biosystems Quantifiler Trio kit to assess the quantity of DNA present along with the male-to-female ratio. This information then guides the decision to use autosomal STR testing with Applied Biosystems Identifiler PCR Amplification Kit or Y-STR testing with Applied Biosystems Yfiler Plus PCR Amplification Kit or both.

Building a forensic DNA analysis lab

Elizabeth Peters, a forensic biologist in the Forensic Science Services division, took on the task of modernizing and upgrading the Fiji DNA Laboratory to ensure the best tools were available to fight crime. Peters describes the efficiencies the laboratory staff experienced when they upgraded their equipment, chemistry and processes to the newest technologies and guidelines available. They have decreased their routine casework turnaround time from a full week to a day.