When Invitrogen™ TOPO™ cloning technology was launched, it sparked a revolution. Over the past 18 years, TOPO technology has become the most widely used cloning method in the world.

TOPO cloning technology is:

  • Efficient—up to 95% of clones contain desired insert
  • Fast—5-minute, room temperature reaction
  • Easy—simple 3-step procedure
  • Proven—over 20,000 citations
  • Flexible—available with or without competent cells, in multiple reaction sizes

No matter what your downstream application is, TOPO cloning technology helps you get the right clone sooner, freeing up your time to answer more important questions.

TOPO cloning technology brochure

Featured video

See how TOPO PCR Cloning technology works

Learn how simple it is to get 95% recovery of your clone in just 5 minutes.

Choose the right TOPO cloning solution for you

For the direct insertion of blunt-end PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Now with 25% extra reactions.

For the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning. Now with 25% extra reactions.

For easy, efficient cloning of long (3–10 kb) PCR products using the Invitrogen™ PCR™-XL-TOPO vector.

TOPO Kits without competent cells

Now with 25% extra reactions.

Gateway Recombinant Cloning with TOPO Technology

Expression cloning with TOPO Technology

Meet the Inventor

Find out how the revolutionary TOPO cloning was developed with Dr. Jon Chestnut, research fellow in Synthetic Biology RND.

Dr. Jon Chesnut
TOPO Cloning Technology

Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications

Cloning Education
Learn about PCR cloning strategies and subcloning approaches

PCR Education
Review the principles of PCR, components, cycling parameters, methods, and applications