Advancing infectious disease surveillance with the MicrobeNet database
The Applied Biosystems™ MicrobeBridge™ software platform is an advanced software solution that seamlessly connects Sanger sequencing results to the Centers for Disease Control and Prevention (CDC) MicrobeNet™ online virtual reference database using 16S sequencing technology. The software enables researchers to more quickly identify microbial pathogens at the root of global outbreaks using a single database curated by subject matter experts.
Currently, analyzing sequencing data is a fragmented process involving multiple software packages. MicrobeBridge Software enables researchers to more easily access the public health information stored in the MicrobeNet database and informs laboratory-based surveillance and the general understanding of disease outbreaks.
MicrobeBridge Software integrates with all Applied Biosystems capillary electrophoresis instruments and automates the assembly and QC of raw Sanger sequencing data into a searchable format in the MicrobeNet database, thus minimizing the effort required to match and positively identify specimens.
Figure 1: MicrobeBridge Software provides an easier view of the overall assembly quality of the sequence and can highlight the discrepancies of the variants for users, QC the assembled sequences, and connect via a single click to the MicrobeNet database for bacterial identification.
Figure 2: Blast amplicon sequence against a library of bacterial 16S rRNA gene sequences to identify the bacterial species using the MicrobeNet database.
- John R. McQuiston, Ph.D., Team Lead, Special Bacteriology Reference Laboratory, Bacterial Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology at the CDC
“Expanding MicrobeNet will allow public health laboratories anywhere in the world to run sequence-based, phenotypic, or eventually other tests and match results against a highly curated database comprised of our unique collection of pathogens. With the addition of MicrobeBridgeTM, researchers and public health laboratories will now be able to easily acquire information on thousands of organisms curated by the pathogen subject matter experts at CDC.”
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