Invitrogen

ALK Polyclonal Antibody

ALK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ALK Antibody (51-3900) in IHC (P)

Immunohistochemistry analysis of ALK showing staining in the cytoplasm of paraffin-embedded human skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperat... View More

ALK Antibody in Immunohistochemistry (IHC)

ALK Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

ALK Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ALK Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

ALK Antibody — IP-MS enrichment of ALK (LFQ intensity): ALK was enriched 568-fold from SR lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and ALK antibody (Product # 51-3900). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

51-3900

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A recombinant protein derived from amino acids 1359-1466 of the 1620 amino acid human ALK protein which corresponds to amino acids 426-528 of the 680 NPM-ALK chimeric protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533907

Target Information

Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aberrant role of ALK in tau proteinopathy through autophagosomal dysregulation.

Molecular psychiatry

Park J,Choi H,Kim YD,Kim SH,Kim Y,Gwon Y,Lee DY,Park SH,Heo WD,Jung YK

51-3900 was used in Immunohistochemistry,Western Blot to propose that aberrantly activated ALK is a bona fide mediator of tau proteinopathy that disrupts autophagosome maturation and causes tau accumulation and aggregation, leading to neuronal dysfunction in AD.

Fri Oct 01 00:00:00 UTC 2021

Aberrant role of ALK in tau proteinopathy through autophagosomal dysregulation.

Molecular psychiatry

Park J,Choi H,Kim YD,Kim SH,Kim Y,Gwon Y,Lee DY,Park SH,Heo WD,Jung YK

Fri Oct 01 00:00:00 UTC 2021

Aberrant role of ALK in tau proteinopathy through autophagosomal dysregulation.

Molecular psychiatry

Park J,Choi H,Kim YD,Kim SH,Kim Y,Gwon Y,Lee DY,Park SH,Heo WD,Jung YK

Fri Oct 01 00:00:00 UTC 2021

Aberrant role of ALK in tau proteinopathy through autophagosomal dysregulation.

Molecular psychiatry

Park J,Choi H,Kim YD,Kim SH,Kim Y,Gwon Y,Lee DY,Park SH,Heo WD,Jung YK

Fri Oct 01 00:00:00 UTC 2021

Anaplastic Lymphoma Kinase Regulates Internalization of the Dopamine D2 Receptor.

Molecular pharmacology

He D,Lasek AW

51-3900 was used in Western Blotting to investigate whether receptor tyrosine kinase anaplastic lymphoma kinase (ALK) regulates dopamine D2 receptor (D2R) internalization.

Sat Feb 01 00:00:00 UTC 2020

ALK oncoproteins in atypical inflammatory myofibroblastic tumours: novel RRBP1-ALK fusions in epithelioid inflammatory myofibroblastic sarcoma.

The Journal of pathology

Lee JC,Li CF,Huang HY,Zhu MJ,Mariño-Enríquez A,Lee CT,Ou WB,Hornick JL,Fletcher JA

513900 was used in western blot to study ALK oncogenes in conventional spindle-cell and typical inflammatory myofibroblastic tumors

Wed Feb 01 00:00:00 UTC 2017

Harnessing Connectivity in a Large-Scale Small-Molecule Sensitivity Dataset.

Cancer discovery

Seashore-Ludlow B,Rees MG,Cheah JH,Cokol M,Price EV,Coletti ME,Jones V,Bodycombe NE,Soule CK,Gould J,Alexander B,Li A,Montgomery P,Wawer MJ,Kuru N,Kotz JD,Hon CS,Munoz B,Liefeld T,Dančík V,Bittker JA,Palmer M,Bradner JE,Shamji AF,Clemons PA,Schreiber SL

Published figure using ALK polyclonal antibody (Product # 51-3900) in Western Blot

Sun Nov 01 00:00:00 UTC 2015

Ethanol activates midkine and anaplastic lymphoma kinase signaling in neuroblastoma cells and in the brain.

Journal of neurochemistry

He D,Chen H,Muramatsu H,Lasek AW

Published figure using ALK polyclonal antibody (Product # 51-3900) in Western Blot

Sun Nov 01 00:00:00 UTC 2015

A novel mechanism of EML4-ALK rearrangement mediated by chromothripsis in a patient-derived cell line.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

Kodama T,Motoi N,Ninomiya H,Sakamoto H,Kitada K,Tsukaguchi T,Satoh Y,Nomura K,Nagano H,Ishii N,Terui Y,Hatake K,Ishikawa Y

51-3900 was used in western blot to examine the mechanism of EML4-ALK rearrangement using a novel cell line

Sat Nov 01 00:00:00 UTC 2014

Selective ALK inhibitor alectinib with potent antitumor activity in models of crizotinib resistance.

Cancer letters

Kodama T,Tsukaguchi T,Yoshida M,Kondoh O,Sakamoto H

51-3900 was used in western blot to assess the efficacy of a selective ALK inhibitor alectinib/CH5424802 in models of crizotinib resistance.

Mon Sep 01 00:00:00 UTC 2014

Wild-type ALK and activating ALK-R1275Q and ALK-F1174L mutations upregulate Myc and initiate tumor formation in murine neural crest progenitor cells.

Oncotarget

Montavon G,Jauquier N,Coulon A,Peuchmaur M,Flahaut M,Bourloud KB,Yan P,Delattre O,Sommer L,Joseph JM,Janoueix-Lerosey I,Gross N,Mühlethaler-Mottet A

51-3900 was used in immunohistochemistry and western blot to study tumor formation in murine neural crest progenitor cells and upregulation of Myc due to wild-type ALK and activating ALK-R1275Q and ALF-F1174L mutations

Mon Jun 30 00:00:00 UTC 2014

Wild-type ALK and activating ALK-R1275Q and ALK-F1174L mutations upregulate Myc and initiate tumor formation in murine neural crest progenitor cells.

Oncotarget

Montavon G,Jauquier N,Coulon A,Peuchmaur M,Flahaut M,Bourloud KB,Yan P,Delattre O,Sommer L,Joseph JM,Janoueix-Lerosey I,Gross N,Mühlethaler-Mottet A

Mon Jun 30 00:00:00 UTC 2014

PDGFR blockade is a rational and effective therapy for NPM-ALK-driven lymphomas.

Nature medicine

Laimer D,Dolznig H,Kollmann K,Vesely PW,Schlederer M,Merkel O,Schiefer AI,Hassler MR,Heider S,Amenitsch L,Thallinger C,Staber PB,Simonitsch-Klupp I,Artaker M,Lagger S,Turner SD,Pileri S,Piccaluga PP,Valent P,Messana K,Landra I,Weichhart T,Knapp S,Shehata M,Todaro M,Sexl V,Höfler G,Piva R,Medico E,Ruggeri BA,Cheng M,Eferl R,Egger G,Penninger JM,Jaeger U,Moriggl R,Inghirami G,Kenner L

51-3900 was used in immunohistochemistry - paraffin section to study the therapeutic potential of PDGFR inhibition in anaplastic large cell lymphoma.

Thu Nov 01 00:00:00 UTC 2012

Antineoplastic activity of the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine in anaplastic large cell lymphoma.

Biochimie

Hassler MR,Klisaroska A,Kollmann K,Steiner I,Bilban M,Schiefer AI,Sexl V,Egger G

Published figure using ALK polyclonal antibody (Product # 51-3900) in Western Blot

Thu Nov 01 00:00:00 UTC 2012

CH5424802, a selective ALK inhibitor capable of blocking the resistant gatekeeper mutant.

Cancer cell

Sakamoto H,Tsukaguchi T,Hiroshima S,Kodama T,Kobayashi T,Fukami TA,Oikawa N,Tsukuda T,Ishii N,Aoki Y

51-3900 was used in western blot to identify and characterize, CH5424802, an inhibitor of anaplastic lymphoma kinase.

Tue May 17 00:00:00 UTC 2011

ALK rearrangement in sickle cell trait-associated renal medullary carcinoma.

Genes, chromosomes & cancer

Mariño-Enríquez A,Ou WB,Weldon CB,Fletcher JA,Pérez-Atayde AR

Published figure using ALK polyclonal antibody (Product # 51-3900) in Western Blot

Tue Mar 01 00:00:00 UTC 2011

Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes.

Osteoarthritis and cartilage

Pufe T,Groth G,Goldring MB,Tillmann B,Mentlein R

51-3900 was used in immunohistochemistry - paraffin section to study the functions of pleiotrophin in cartilage

Thu Feb 01 00:00:00 UTC 2007

Anaplastic lymphoma kinase is activated through the pleiotrophin/receptor protein-tyrosine phosphatase beta/zeta signaling pathway: an alternative mechanism of receptor tyrosine kinase activation.

The Journal of biological chemistry

Perez-Pinera P,Zhang W,Chang Y,Vega JA,Deuel TF

Published figure using ALK polyclonal antibody (Product # 51-3900) in Immunocytochemistry

Fri Sep 28 00:00:00 UTC 2007

Anaplastic lymphoma kinase is activated through the pleiotrophin/receptor protein-tyrosine phosphatase beta/zeta signaling pathway: an alternative mechanism of receptor tyrosine kinase activation.

The Journal of biological chemistry

Perez-Pinera P,Zhang W,Chang Y,Vega JA,Deuel TF

Published figure using ALK polyclonal antibody (Product # 51-3900) in Immunocytochemistry

Fri Sep 28 00:00:00 UTC 2007

Anaplastic lymphoma kinase is activated through the pleiotrophin/receptor protein-tyrosine phosphatase beta/zeta signaling pathway: an alternative mechanism of receptor tyrosine kinase activation.

The Journal of biological chemistry

Perez-Pinera P,Zhang W,Chang Y,Vega JA,Deuel TF

Published figure using ALK polyclonal antibody (Product # 51-3900) in Immunocytochemistry

Fri Sep 28 00:00:00 UTC 2007

Stat3 is required for ALK-mediated lymphomagenesis and provides a possible therapeutic target.

Nature medicine

Chiarle R,Simmons WJ,Cai H,Dhall G,Zamo A,Raz R,Karras JG,Levy DE,Inghirami G

Wed Jun 01 00:00:00 UTC 2005

Stat3 is required for ALK-mediated lymphomagenesis and provides a possible therapeutic target.

Nature medicine

Chiarle R,Simmons WJ,Cai H,Dhall G,Zamo A,Raz R,Karras JG,Levy DE,Inghirami G

Wed Jun 01 00:00:00 UTC 2005

Stat3 is required for ALK-mediated lymphomagenesis and provides a possible therapeutic target.

Nature medicine

Chiarle R,Simmons WJ,Cai H,Dhall G,Zamo A,Raz R,Karras JG,Levy DE,Inghirami G

Wed Jun 01 00:00:00 UTC 2005

Stat3 is required for ALK-mediated lymphomagenesis and provides a possible therapeutic target.

Nature medicine

Chiarle R,Simmons WJ,Cai H,Dhall G,Zamo A,Raz R,Karras JG,Levy DE,Inghirami G

Wed Jun 01 00:00:00 UTC 2005

Truncated ALK derived from chromosomal translocation t(2;5)(p23;q35) binds to the SH3 domain of p85-PI3K.

Mutation research

Polgar D,Leisser C,Maier S,Strasser S,Rüger B,Dettke M,Khorchide M,Simonitsch I,Cerni C,Krupitza G

51-3900 was used in western blot to investigate the molecular interaction between ALK and the regulatory p85 subunit of PI3K

Tue Feb 15 00:00:00 UTC 2005

Differential induction of glioblastoma migration and growth by two forms of pleiotrophin.

The Journal of biological chemistry

Lu KV,Jong KA,Kim GY,Singh J,Dia EQ,Yoshimoto K,Wang MY,Cloughesy TF,Nelson SF,Mischel PS

Fri Jul 22 00:00:00 UTC 2005

Differential induction of glioblastoma migration and growth by two forms of pleiotrophin.

The Journal of biological chemistry

Lu KV,Jong KA,Kim GY,Singh J,Dia EQ,Yoshimoto K,Wang MY,Cloughesy TF,Nelson SF,Mischel PS

Fri Jul 22 00:00:00 UTC 2005

NPM-ALK transgenic mice spontaneously develop T-cell lymphomas and plasma cell tumors.

Blood

Chiarle R,Gong JZ,Guasparri I,Pesci A,Cai J,Liu J,Simmons WJ,Dhall G,Howes J,Piva R,Inghirami G

Sat Mar 01 00:00:00 UTC 2003

NPM-ALK transgenic mice spontaneously develop T-cell lymphomas and plasma cell tumors.

Blood

Chiarle R,Gong JZ,Guasparri I,Pesci A,Cai J,Liu J,Simmons WJ,Dhall G,Howes J,Piva R,Inghirami G

Sat Mar 01 00:00:00 UTC 2003

NPM-ALK transgenic mice spontaneously develop T-cell lymphomas and plasma cell tumors.

Blood

Chiarle R,Gong JZ,Guasparri I,Pesci A,Cai J,Liu J,Simmons WJ,Dhall G,Howes J,Piva R,Inghirami G

Sat Mar 01 00:00:00 UTC 2003

Anaplastic lymphoma kinase (ALK) activates Stat3 and protects hematopoietic cells from cell death.

Oncogene

Zamo A,Chiarle R,Piva R,Howes J,Fan Y,Chilosi M,Levy DE,Inghirami G

Thu Feb 07 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; p80; TFG/ALK

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Gene Aliases: ALK; CD246; NBLST3

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UniProt ID: (Human) Q9UM73

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Entrez Gene ID: (Human) 238

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Function(s)

NF-kappaB-inducing kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding identical protein binding transmembrane signal receptor

Process(es)

activation of MAPK activity signal transduction transmembrane receptor protein tyrosine kinase signaling pathway cell proliferation phosphorylation peptidyl-tyrosine phosphorylation NIK/NF-kappaB signaling regulation of apoptotic process protein autophosphorylation neuron development positive regulation of NF-kappaB transcription factor activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ALK Polyclonal Antibody

ALK Antibody (51-3900) in IHC (P)

Product Details

51-3900

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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