As every researcher knows, no single application or technique tells the whole story. Often, it is the thoughtful combination of approaches that delivers the insight needed to move research forward. We have interviewed three researchers who are successfully employing various capillary electrophoresis (CE) applications to fill in some of the gaps or ambiguities in their next-generation sequencing (NGS) data. Read "Case studies in complementary capillary electrophoresis and next-generation applications" to find more about the utility of combining NGS and CE data to gain a more complete picture.

sanger sequencing

Sanger Sequencing for Mutation Analysis

Sanger sequencing provides fast and accurate analysis of the mutations present at a frequency of 20% or greater in a sample. The combination of the new BigDye® Direct Cycle Sequencing Kit and a 3500 Series Genetic Analyzer provide the most comprehensive and reliable sequencing data.

Learn more about BigDye® Direct Cycle Sequencing kit

Fragment Analysis

Fragment Analysis for Somatic Mutation Detection

Detection of somatic mutations in samples like formalin-fixed, paraffin-embedded (FFPE) tissues may require higher levels of sensitivity and specificity than can be achieved with Sanger sequencing approaches. Fragment analysis provides solutions for achieving the high levels of sensitivity and specificity needed for somatic mutation detection.

Sanger Sequencing for Genome Finishing

Sanger Sequencing for Genome Finishing

Sanger sequencing can perform in many situations where next-generation sequencing is limited, such as sequencing through difficult homopolymeric regions. Use the 3500 Genetic Analyzer for gap closure and to fill in regions of sequences that cannot be identified by next-generation sequencing approaches.

Download "Resolving homopolymeric ambiguities using PCR-based fragment analysis” application note

Sanger Sequencing for Genome Finishing

Primer Designer Tool

This fully automated online resource lets you quickly search for, configure and order primers for the Sanger confirmation step of your NGS workflow. The database consists of ~650,000 pre-designed primer pairs for re-sequencing the human exome and human mitochondrial genome.

Learn more about Primer Designer Tool


Review our comprehensive list of publications describing Sanger sequencing and fragment analysis applications used in conjunction with next-generation sequencing.

The Sanger Sequencing Publication List

Showing of | Show All

Sort by: