GORK Antibodies

Target Information

GORK encodes a plant Shaker-type, voltage-gated K+ channel that localizes predominantly to the plasma membrane of guard cells (and is also detected in peripheral puncta consistent with membrane microdomain clustering), where it provides the major outward-rectifying K+ conductance enabling K+ efflux during stomatal closure. The channel is built as a tetramer of pore-forming subunits; each subunit contains an N-terminal cytosolic region, six transmembrane helices (S1-S6) with a canonical re-entrant pore loop between S5 and S6 that confers high K+ selectivity, and a cytosolic C-terminal regulatory region. Recent structural work shows that the C terminus includes a C-linker coupled to a cyclic nucleotide-binding homology domain (CNBHD) and an ankyrin-repeat domain, with conformational rearrangements across this cytosolic module proposed to tune gating. Functionally, GORK mediates depolarization-activated, outwardly rectifying K+ currents and exhibits K+-dependent gating, properties that help ensure net K+ release when the electrochemical driving force favors efflux; loss-of-function or dominant-negative interference suppresses guard-cell outward K+ currents and perturbs stomatal movement and transpiration control.

Synonyms

AT5G37500; gated outwardly-rectifying K+ channel; MPA22.4; MPA22_4