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Invitrogen
Phosphotyrosine Monoclonal Antibody (pY20), HRP
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FIGURE: 1 / 3
Phosphotyrosine Antibody (03-7720) in ELISA
Direct ELISA analysis of Phosphotyrosine was performed by coating wells of a 96-well plate with 100 µL per well of Phosphotyrosine or Phosphoserine diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 5 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100 µL per well of a HRP-conjugated anti-phosphotyrosine mouse monoclonal antibody (Product # 03-7720) at a starting concentration of 1 µg/mL and serially diluting 2-fold to a con... View More
Product Details
03-7720
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
pY20
Immunogen
Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4, with 40% glycerol, 4mg/mL BSA
Contains
0.19% Kathon™ CG
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
03-7720 has been successfully used in ELISA and WB applications, and detects proteins which contain phosphorylated tyrosine residues and proteins containing phosphorylated threonine residues.
Target Information
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Phosphotyrosine; pTyr; pY
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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