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CD54 (ICAM-1) Monoclonal Antibody (HA58), PE, eBioscience™
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FIGURE: 1 / 12
CD54 (ICAM-1) Antibody (12-0549-42) in Flow
Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells with0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE,eBioscience™ (Product # 12-4714-82, yellow histogram) or0.25 µgCD19 Monoclonal Antibody (HIB19), PE,eBioscience™ (Product # 12-0549-42, blue histogram).Raji Cas9 control cells were also stained with0... View More
Product Details
12-0549-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Bacteria,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HA58
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.
Applications Reported: The HA58 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The HA58 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; Human rhinovirus receptor; ICAM-1; ICAM-1 soluble variant; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1 precursor; Ly 47; Major group rhinovirus receptor; major group rhinovirus receptor precusor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells; unnamed protein product
Gene Aliases: BB2; CD54; ICAM1; P3.58
UniProt ID: (Human) P05362
Entrez Gene ID: (Human) 3383
regulation of leukocyte mediated cytotoxicity
immune effector process
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
T cell antigen processing and presentation
positive regulation of cellular extravasation
immune response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
membrane to membrane docking
cell adhesion mediated by integrin
adhesion of symbiont to host
viral entry into host cell
receptor-mediated virion attachment to host cell
leukocyte migration
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to glucose stimulus
T cell extravasation
cell-cell adhesion
regulation of ruffle assembly
cellular response to oxygen-containing compound
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
cellular response to beta-amyloid
cellular response to leukemia inhibitory factor
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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