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Invitrogen
Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), Biotin, eBioscience™
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FIGURE: 1 / 40
Galectin 3 Antibody (13-5301-82) in Flow
Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC (Product # 17-0112-82) and 0.125 µg of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Product Details
13-5301-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioM3/38 (M3/38)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.
This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).
Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Staining can be done using Foxp3 buffers () or IC Fixation Buffers (Product # 88-8823-88).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; epididymis secretory sperm binding protein; epithelial-specific lectin; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS3; MAC-2 antigen; unnamed protein product
Gene Aliases: CBP35; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2
UniProt ID: (Human) P17931
Entrez Gene ID: (Human) 3958, (Mouse) 16854
RNA binding
protein phosphatase inhibitor activity
protein binding
IgE binding
protein phosphatase binding
carbohydrate binding
receptor inhibitor activity
chemoattractant activity
laminin binding
disaccharide binding
oligosaccharide binding
molecular condensate scaffold activity
receptor ligand inhibitor activity
Fc-gamma receptor I complex binding
poly(A) RNA binding
monosaccharide binding
advanced glycation end-product receptor activity
extracellular matrix protein
immune system process
monocyte chemotaxis
mRNA processing
RNA splicing
cell differentiation
neutrophil chemotaxis
epithelial cell differentiation
positive regulation of protein complex assembly
killing of cells of other organism
regulation of T cell proliferation
innate immune response
negative regulation of endocytosis
eosinophil chemotaxis
macrophage chemotaxis
negative regulation of T cell receptor signaling pathway
positive chemotaxis
NK T cell activation
negative regulation of NK T cell activation
antimicrobial humoral immune response mediated by antimicrobial peptide
regulation of T cell apoptotic process
mononuclear cell migration
positive regulation of mononuclear cell migration
positive regulation of calcium ion import
regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of protein localization to plasma membrane
negative regulation of immunological synapse formation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
positive regulation of dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
skeletal system development
signal transduction
positive regulation of cell proliferation
positive regulation of serotonin secretion
extracellular matrix organization
negative regulation of apoptotic process
positive regulation of angiogenesis
negative regulation of cell proliferation in bone marrow
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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