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Invitrogen
Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™
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FIGURE: 1 / 46
Galectin 3 Antibody (14-5301-82) in ICC/IF
Immunofluorescence analysis of Galectin 3 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™ (Product # 14-5301-85) at 1:100 dilut... View More
Product Details
14-5301-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioM3/38 (M3/38)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.
This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).
Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections, and ELISA.
Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; MAC-2 antigen
Gene Aliases: CBP35; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2
UniProt ID: (Human) P17931
Entrez Gene ID: (Human) 3958, (Mouse) 16854
monocyte chemotaxis
mRNA processing
RNA splicing
neutrophil chemotaxis
epithelial cell differentiation
regulation of T cell proliferation
innate immune response
negative regulation of endocytosis
eosinophil chemotaxis
macrophage chemotaxis
negative regulation of T cell receptor signaling pathway
positive chemotaxis
regulation of T cell apoptotic process
mononuclear cell migration
positive regulation of mononuclear cell migration
positive regulation of calcium ion import
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of immunological synapse formation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
positive regulation of dendritic cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
skeletal system development
immune system process
signal transduction
positive regulation of cell proliferation
positive regulation of serotonin secretion
cell differentiation
extracellular matrix organization
negative regulation of apoptotic process
positive regulation of angiogenesis
negative regulation of cell proliferation in bone marrow
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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