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Invitrogen

Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), PE, eBioscience™

4 Published Figures
5 References
View all (112) Galectin 3 antibodies

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Product Details

12-5301-82

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test
View 4 publications 4 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioM3/38 (M3/38)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_842792

Product Specific Information

Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).

Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; epididymis secretory sperm binding protein; epithelial-specific lectin; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS3; MAC-2 antigen; unnamed protein product

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Gene Aliases: CBP35; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2

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UniProt ID: (Human) P17931

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Entrez Gene ID: (Human) 3958, (Mouse) 16854

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Function(s)
RNA binding protein phosphatase inhibitor activity protein binding IgE binding protein phosphatase binding carbohydrate binding receptor inhibitor activity chemoattractant activity laminin binding disaccharide binding oligosaccharide binding molecular condensate scaffold activity receptor ligand inhibitor activity Fc-gamma receptor I complex binding poly(A) RNA binding monosaccharide binding advanced glycation end-product receptor activity extracellular matrix protein
Process(es)
immune system process monocyte chemotaxis mRNA processing RNA splicing cell differentiation neutrophil chemotaxis epithelial cell differentiation positive regulation of protein complex assembly killing of cells of other organism regulation of T cell proliferation innate immune response negative regulation of endocytosis eosinophil chemotaxis macrophage chemotaxis negative regulation of T cell receptor signaling pathway positive chemotaxis NK T cell activation negative regulation of NK T cell activation antimicrobial humoral immune response mediated by antimicrobial peptide regulation of T cell apoptotic process mononuclear cell migration positive regulation of mononuclear cell migration positive regulation of calcium ion import regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to plasma membrane negative regulation of immunological synapse formation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of dendritic cell differentiation negative regulation of extrinsic apoptotic signaling pathway skeletal system development signal transduction positive regulation of cell proliferation positive regulation of serotonin secretion extracellular matrix organization negative regulation of apoptotic process positive regulation of angiogenesis negative regulation of cell proliferation in bone marrow
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