Contains Sodium azide and formaldehyde
Invitrogen™

eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)

Catalog number:  88-8823-88

Related applications:

Cellular Imaging

| Flow Cytometry

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Description

The eBioscience Fixation and Permeabilization Kit is designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture – to cause accumulation of cytokine protein at the Golgi and enhance resulting staining. A fixation buffer is used to cross link, stabilize, and fix the cell membrane, while the permeabilization buffer is used to reversibly permeabilize the cell membrane so the staining antibodies can enter the cell effectively.

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: eBioscience™
Reagent Type: Fixation Reagent, Permeabilization Reagent