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GM-CSF Monoclonal Antibody (MP1-22E9), APC, eBioscience™
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FIGURE: 1 / 3
GM-CSF Antibody (17-7331-80) in Flow
eBioscience™ Mouse Cytokine Positive Control Cells (Product # 00-4500-51) were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-81) (left) or 0.25 µg of GM-CSF Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis.
Product Details
17-7331-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MP1-22E9
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.
Applications Reported: This MP1-22E9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MP1-22E9 antibody has been tested by intracellular staining followed by flow cytometric analysis of restimulated mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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