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CD203c Monoclonal Antibody (NP4D6), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 1
CD203c Antibody (25-2039-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD123 FITC (Product # 11-1239-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD203c PE-Cyanine7 (right). Cells in the small scatter population were used for analysis.
Product Details
25-2039-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
NP4D6
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This NP4D6 monoclonal antibody reacts with human CD203c, which is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3). ENPP family members catalyze the hydrolysis of pyrophosphate and phosphodiester bonds to nucleoside 5-monophosphates. A type II transmembrane protein, CD203c is expressed on basophils and mast cells. CD203c expression is upregulated on basophils activated by allergen or IgE crosslinking. Therefore, CD203c is used as an indicator of basophil activation in various IgE-mediated allergic responses.
Applications Reported: This NP4D6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This NP4D6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. [provided by RefSeq, Jul 2008]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alkaline phosphodiesterase I; CD203c; Dinucleoside polyphosphatase; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); E-NPP 3; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; ENPP3; gp130RB13-6; NPPase; Nucleotide diphosphatase; Nucleotide pyrophosphatase; PD-Ibeta; Phosphodiesterase I beta; Phosphodiesterase I/nucleotide pyrophosphatase 3; phosphodiesterase-I beta; unnamed protein product
Gene Aliases: B10; CD203c; ENPP3; NPP3; PD-IBETA; PDNP3
UniProt ID: (Human) O14638
Entrez Gene ID: (Human) 5169
nucleic acid binding
bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity
phosphodiesterase I activity
calcium ion binding
zinc ion binding
pyrophosphatase activity
hydrolase activity
bis(5'-adenosyl)-pentaphosphatase activity
GTP diphosphatase activity
UTP diphosphatase activity
metal ion binding
nucleoside-triphosphate diphosphatase activity
ATP diphosphatase activity
bis(5'-adenosyl)-triphosphatase activity
basophil activation involved in immune response
pyrimidine nucleotide metabolic process
phosphate-containing compound metabolic process
nucleoside triphosphate catabolic process
inorganic diphosphate transport
negative regulation of mast cell activation involved in immune response
ATP metabolic process
negative regulation of inflammatory response
phosphate ion homeostasis
negative regulation of mast cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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