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Invitrogen
CXCL13 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CXCL13 Antibody (500-P141BT-50UG) in WB
Western Blot: To detect hBCA-1 by Western Blot analysis CXCL13 Polyclonal Antibody, Biotin (Product # 500-P141BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBCA-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P141BT-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BCA-1 (CXCL13)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BCA-1 (CXCL13). Anti-Human BCA-1 (CXCL13)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human BCA-1 (CXCL13) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BCA-1 (CXCL13) (500-P141) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BCA-1 (CXCL13).
Western Blot: To detect hBCA-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBCA-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P141BT-1MG will be provided as 2 x 500 µg
Target Information
CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt's Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Angie; B cell-attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); B-lymphocyte chemoattractant; BCA-1; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 13; CC motif chemokine; CCmotif chemokine; chem; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); CXC; cxc chemokine; CXC chemokine BLC; CXC motif chemokine; CXC motif chemokine 13; CXCL; CXCL13; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); Small-inducible cytokine B13
Gene Aliases: ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; CXCL13; SCYB13
UniProt ID: (Human) O43927
Entrez Gene ID: (Human) 10563
germinal center formation
lymphocyte chemotaxis across high endothelial venule
chronic inflammatory response
regulation of humoral immune response
chemotaxis
defense response
inflammatory response
immune response
cell surface receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
positive regulation of T cell chemotaxis
neutrophil chemotaxis
killing of cells of other organism
regulation of Rap protein signal transduction
positive regulation of integrin activation
positive regulation of cell-cell adhesion mediated by integrin
B cell chemotaxis
endothelial cell chemotaxis to fibroblast growth factor
defense response to bacterium
regulation of angiogenesis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
negative regulation of endothelial cell chemotaxis to fibroblast growth factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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