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Invitrogen
NNT1 Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
NNT1 Antibody (500-P186-50UG) in WB
Western Blot: To detect Human NNT-1/BCSF-3 by Western Blot analysis NNT1 Polyclonal Antibody (Product # 500-P186-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P186-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human NNT-1/BCSF-3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NNT-1/BCSF-3. Anti-Human NNT-1/BCSF-3-specific antibody was purified by affinity chromatography employing an immobilized Human NNT-1/BCSF-3 matrix.
Sandwich ELISA: To detect Human NNT-1/BCSF-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NNT-1/BCSF-3 (500-P186Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NNT-1/BCSF-3.
Western Blot: To detect Human NNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
Target Information
NNT-1/BCSF-3 is a neurotrophic factor with B-cell-stimulating capabilities. Expressed in the lymph nodes and spleen, NNT-1/BCSF-3 activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b) by binding and inducing the tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss, and B cell hyperplasia associated with increases in serum IgG and IgM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell stimulatory factor 3; B-cell-stimulating factor 3; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; novel neurotrophin-1; unnamed protein product
Gene Aliases: BSF-3; BSF3; CISS2; CLC; NNT-1; NNT1; NR6
UniProt ID: (Human) Q9UBD9
Entrez Gene ID: (Human) 23529
positive regulation of immunoglobulin production
cell surface receptor signaling pathway
JAK-STAT cascade
positive regulation of cell proliferation
cytokine-mediated signaling pathway
B cell differentiation
positive regulation of B cell proliferation
negative regulation of neuron apoptotic process
positive regulation of isotype switching to IgE isotypes
positive regulation of astrocyte differentiation
receptor signaling pathway via STAT
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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