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Invitrogen
Biotin Monoclonal Antibody (BK-1/39), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 4
Biotin Antibody (53-9895-82) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0036-42) and Anti-Human CD4 Biotin (Product # 13-0048-82) followed by either 0.125 µg of Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or 0.125 µg of Anti-Biotin Alexa Fluor® 488 (Product # 53-9895-82) (right). Cells in the lymphocyte gate were used for analysis.
Product Details
53-9895-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
BK-1/39
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This BK-1/39 monoclonal antibody reacts with biotin, a B-complex vitamin. As a coenzyme, biotin is involved in the metabolism of fatty acids, amino acids, and gluconeogenesis. In the laboratory, biotin is used to tag molecules of interest for biochemical or cell biological studies. This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired.
Applications Reported: This BK-1/39 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This BK-1/39 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with a biotin-conjugated antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Vitamin B4
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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