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Invitrogen
P2Y12 Recombinant Rabbit Monoclonal Antibody (4H5L19)
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FIGURE: 1 / 6
P2Y12 Antibody (702516) in ICC/IF
Immunofluorescent analysis of P2Y12 (red) in human iPSC-derived microglia. The cells were fixed with 4% paraformaldehyde for 15 min at room temperature, and then permeabilized and blocked with 0.25% Triton X-100 and 10% donkey serum in PBS for 20 min. Samples were then incubated with Anti-P2Y12 Recombinant Rabbit Monoclonal Antibody (red; Product # 702516) at a dilution of 1:500 in PBS containing 0.25% Triton X-100 and 10% donkey serum at 48304C overnight, followed by incubation with Donkey Anti-Rabbit Alexa Fluor 568 (Product # A10042) at... View More
Product Details
702516
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4H5L19
Immunogen
Peptides corresponding to human P2Y12 [1) aa323-aa342, 2) aa119-aa138]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Dog
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
P2Y12 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelets aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Two transcript variants encoding the same isoform have been identified for this gene. Expression of P2Y12 has been reported in platelets, spinal cord, and many brain regions. ESTs for P2Y12 have been isolated from B-cell/lung/testis, brain, embryo, prostate, eye, kidney carcinoma, and colon carcinoma libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADP-glucose receptor; ADPG-R; G protein-coupled receptor; G-protein coupled receptor SP1999; Gi-coupled ADP receptor HORK3; P2T(AC); P2Y purinoceptor 12; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; P2Y12 platelet ADP receptor; P2YR12; purinergic receptor P2RY12; purinergic receptor P2Y, G-protein coupled, 12; putative G-protein coupled receptor; SP1999; unnamed protein product
Gene Aliases: 2900079B22Rik; 4921504D23Rik; ADPG-R; BDPLT8; HORK3; P2RY12; P2T(AC); P2Y(12)R; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999
UniProt ID: (Human) Q9H244, (Mouse) Q9CPV9, (Rat) Q9EPX4
Entrez Gene ID: (Human) 64805, (Mouse) 70839, (Rat) 64803
adenosine receptor signaling pathway
ion transport
substrate-dependent cell migration, cell extension
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
blood coagulation
hemostasis
calcium-mediated signaling
cerebral cortex radial glia guided migration
cell projection organization
lamellipodium assembly
platelet activation
positive regulation of integrin activation by cell surface receptor linked signal transduction
positive regulation of cell adhesion mediated by integrin
G-protein coupled purinergic nucleotide receptor signaling pathway
positive regulation of ion transport
response to axon injury
regulation of chemotaxis
positive regulation of chemotaxis
establishment of localization in cell
positive regulation of protein kinase B signaling
platelet aggregation
cellular response to ATP
visual system development
positive regulation of ruffle assembly
regulation of microglial cell migration
positive regulation of microglial cell migration
glial cell migration
negative regulation of norepinephrine secretion
protein kinase B signaling
negative regulation of cell differentiation
regulation of calcium ion transport
calcium ion transmembrane transport
cellular response to organic cyclic compound
potassium ion transmembrane transport
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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