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PCNA Antibody (752-9910-82) in IHC (P)
Immunohistochemical analysis of PCNA was performed on FFPE human colon tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 10 mins, followed by a 5-min cool down and a 5-min wash with ddH2O. Tissues were permeabilized with 0.1% Triton X-100 in 1X PBS for 30 mins and blocked with 3% BSA/5% normal goat serum/1X PBS for 1 hr at... View More
Product Details
752-9910-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
PC10
Immunogen
Rat recombinant PCNA polypeptides.
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The PC10 antibody reacts with human PCNA which is involved in the control of eukaryotic DNA replication and found in the nucleus.
Applications Reported: This PC10 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This PC10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; DNA sliding clamp PCNA; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen; unnamed protein product
Gene Aliases: ATLD2; PCNA
UniProt ID: (Human) P12004
Entrez Gene ID: (Human) 5111
purine-specific mismatch base pair DNA N-glycosylase activity
DNA binding
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
macromolecular complex binding
DNA polymerase binding
negative regulation of transcription from RNA polymerase II promoter
DNA replication
leading strand elongation
regulation of DNA replication
DNA repair
base-excision repair, gap-filling
mismatch repair
chromatin organization
cellular response to DNA damage stimulus
response to oxidative stress
heart development
translesion synthesis
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
response to estradiol
response to lipid
cellular response to UV
estrous cycle
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
cellular response to hydrogen peroxide
cellular response to xenobiotic stimulus
response to dexamethasone
liver regeneration
positive regulation of DNA-directed DNA polymerase activity
response to L-glutamate
mitotic telomere maintenance via semi-conservative replication
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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