Invitrogen

ACAA2 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

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ACAA2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of ACAA2 in human cell line CACO-2 shows positivity in mitochondria. Samples were probed using an ACAA2 Polyclonal Antibody (Product # PA5-59942).

ACAA2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of ACAA2 in human kidney and pancreas tissues using ACAA2 Polyclonal Antibody (Product # PA5-59942). Corresponding ACAA2 RNA-seq data are presented for the same tissues.

ACAA2 Antibody in Western Blot (WB) — Western blot analysis of ACAA2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ACAA2 Polyclonal Antibody (Product # PA5-59942).

ACAA2 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ACAA2 demonstrates antibody specificity. Immunohistochemical analysis of ACAA2 using anti-ACAA2 Polyclonal Antibody (Product # PA5-59942), shows significant staining of ACAA2 in human kidney and shows minimal or weak staining in human pancreas tissues. The relative expression levels of ACAA2 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-59942

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:50	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human ACAA2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.05 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2637576

Product Specific Information

Immunogen sequence: LLRGVFVVAA KRTPFGAYGG LLKDFTATDL SEFAAKAALS AGKVSPETVD SVIMGNVLQS SSDAIYLARH VGLRVGIPKE TPALTINRLC GSGFQSIVNG CQEICVKEAE VVLCGGTESM SQAPYCVRNV RFGTKLGSDI KLEDSLW

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 86%.

Target Information

ACAA2 (3-Ketoacyl-CoA thiolase) is an enzyme that in humans is encoded by the ACAA2 gene. Acetyl-Coenzyme A acyltransferase 2 is an acetyl-CoA C-acyltransferase enzyme. The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-ketoacyl-CoA thiolase, mitochondrial; Acetyl-CoA acetyltransferase; Acetyl-CoA acyltransferase; acetyl-Coenzyme A acyltransferase 2; acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase); Acyl-CoA hydrolase, mitochondrial; beta ketothiolase; Beta-ketothiolase; Mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase; T1

Gene Aliases: 0610011L04Rik; ACAA2; AI255831; AI265397; D18Ertd240e; DSAEC

UniProt ID: (Human) P42765, (Mouse) Q8BWT1, (Rat) P13437

Entrez Gene ID: (Human) 10449, (Mouse) 52538, (Rat) 170465

Molecular Function: acyltransferase