Immunofluorescent analysis of ADHFE1 showing staining in the mitochondria of Hep G2 cells. Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and stained using an ADHFE1 polyclonal antibody (Product # PA5-31415) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 27 and 257 of Human ADHFE1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31415 targets ADHFE1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-31415 immunogen is recombinant fragment corresponding to a region within amino acids 27 and 257 of Human ADHFE1.
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 220.127.116.11), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.