Invitrogen

ALG1 Polyclonal Antibody

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Antibody Testing Data (1)
Published Figures (1)

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ALG1 Antibody (PA1-031) in WB

Western blot analysis of Alg-1 was performed by loading 15 µg WT or Alg-1 (-) worm extract onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Alg-1 (Product # PA1-031) at a dilution of 1:5000 for 1 hour and room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution o... View More

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ALG1 Antibody in Immunoprecipitation (IP) — Published figure using ALG1 polyclonal antibody (Product # PA1-031) in Immunoprecipitation

Product Details

PA1-031

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 3 publications 3 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

C. elegans

Published species

C. elegans

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence DLEEIPNSPPTQPQTFSDVPQR

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2539852

Target Information

The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An optimized protocol for isolation of S-nitrosylated proteins from C. elegans.

STAR protocols

Seth P,Premont RT,Stamler JS

PA1-031 was used in Western Blot to describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from C. elegans, suitable for assessing SNO levels of individual proteins and of the global proteome.

Fri Jun 18 00:00:00 EDT 2021

A microRNA feedback loop regulates global microRNA abundance during aging.

RNA (New York, N.Y.)

Inukai S,Pincus Z,de Lencastre A,Slack FJ

PA1-031 was used in Western Blotting to investigate the network of genes that controls miRNA transcription and processing during Caenorhabditis elegans ageing.

Thu Feb 01 00:00:00 EST 2018

Detection of microRNA-Target Interactions by Chimera PCR (ChimP).

Methods in molecular biology (Clifton, N.J.)

Broughton JP,Pasquinelli AE

PA1-031 was used in Sample Preparation (PCR) to describe a method, called Chimera PCR (ChimP), for the validation or testing of specific miRNA-target interactions.

Thu Mar 21 00:00:00 EDT 2019

A novel function for the DEAD-box RNA helicase DDX-23 in primary microRNA processing in Caenorhabditis elegans.

Developmental biology

Chu YD,Chen HK,Huang T,Chan SP

PA1-031X was used in western blot to identify DEAD/H-box proteins involved in miRNA biogenesis

Fri Jan 15 00:00:00 EST 2016

Identifying Argonaute binding sites in Caenorhabditis elegans using iCLIP.

Methods (San Diego, Calif.)

Broughton JP,Pasquinelli AE

PA1-031 was used in Western Blotting to identify Argonaute binding sites in Caenorhabditis elegans using individual nucleotide resolution crosslinking and immunoprecipitation.

Sun Sep 15 00:00:00 EDT 2013

Identifying Argonaute binding sites in Caenorhabditis elegans using iCLIP.

Methods (San Diego, Calif.)

Broughton JP,Pasquinelli AE

PA1-031 was used in Western Blotting to identify Argonaute binding sites in Caenorhabditis elegans using individual nucleotide resolution crosslinking and immunoprecipitation.

Sun Sep 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Argonaute; Argonaute-like

Gene Aliases: CELE_F48F7.1

Entrez Gene ID: (C. elegans) 181504

Function(s)

nematode larval development embryo development ending in birth or egg hatching gene silencing by RNA production of miRNAs involved in gene silencing by miRNA mRNA cleavage involved in gene silencing by miRNA vulval development regulation of development, heterochronic hermaphrodite genitalia development regulation of cell fate specification small RNA loading onto RISC RNA phosphodiester bond hydrolysis, endonucleolytic

Process(es)

nucleic acid binding double-stranded RNA binding single-stranded RNA binding endoribonuclease activity, producing 3'-phosphomonoesters miRNA binding endoribonuclease activity, cleaving siRNA-paired mRNA

ALG1 Polyclonal Antibody

ALG1 Antibody (PA1-031) in WB

Product Details

PA1-031

Western Blot (WB)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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