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          • Primary Antibodies ›
          • ALG1 Antibodies

          Invitrogen

          ALG1 Polyclonal Antibody

          2 Published Figures
          8 References
          View all (8) ALG1 antibodies

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          Cite ALG1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (2)
          ALG1 Antibody in Western Blot (WB)
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          ALG1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          ALG1 Antibody (PA1-031) in WB

          Western blot analysis of Alg-1 was performed by loading 15 µg WT or Alg-1 (-) worm extract onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Alg-1 (Product # PA1-031) at a dilution of 1:5000 for 1 hour and room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution o... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          ALG1 Antibody in Western Blot (WB)
          ALG1 Antibody in Western Blot (WB)
          ALG1 Antibody in Immunoprecipitation (IP)

          Product Details

          PA1-031

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:5,000
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (Misc)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          C. elegans

          Published species

          C. elegans

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide sequence DLEEIPNSPPTQPQTFSDVPQR

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA, 30% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2539852

          Target Information

          The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Argonaute; Argonaute-like

          View more View less

          Gene Aliases: CELE_F48F7.1

          View more View less

          Entrez Gene ID: (C. elegans) 181504

          View more View less

          Function(s)
          nucleic acid binding double-stranded RNA binding single-stranded RNA binding endoribonuclease activity, producing 3'-phosphomonoesters miRNA binding endoribonuclease activity, cleaving siRNA-paired mRNA
          Process(es)
          nematode larval development embryo development ending in birth or egg hatching gene silencing by RNA production of miRNAs involved in gene silencing by miRNA mRNA cleavage involved in gene silencing by miRNA vulval development regulation of development, heterochronic hermaphrodite genitalia development regulation of cell fate specification small RNA loading onto RISC RNA phosphodiester bond hydrolysis, endonucleolytic
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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