Invitrogen

ALG1 Polyclonal Antibody

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ALG1 Antibody in Western Blot (WB) — Western blot analysis of Alg-1 was performed by loading 15 µg WT or Alg-1 (-) worm extract onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Alg-1 (Product # PA1-031) at a dilution of 1:5000 for 1 hour and room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product # 34077).

ALG1 Antibody in Immunoprecipitation (IP) — Published figure using ALG1 polyclonal antibody (Product # PA1-031) in Immunoprecipitation

Product Details

PA1-031

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000	View 3 publications 3 publications
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	C. elegans
Published species	C. elegans
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide sequence DLEEIPNSPPTQPQTFSDVPQR
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA, 30% glycerol
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2539852

Target Information

The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An optimized protocol for isolation of S-nitrosylated proteins from C. elegans.

STAR protocols

Seth P,Premont RT,Stamler JS

PA1-031 was used in Western Blot to describe a protocol for isolating S-nitrosylated proteins (SNO-proteins) from C. elegans, suitable for assessing SNO levels of individual proteins and of the global proteome.

Fri Jun 18 00:00:00 EDT 2021

A microRNA feedback loop regulates global microRNA abundance during aging.

RNA (New York, N.Y.)

Inukai S,Pincus Z,de Lencastre A,Slack FJ

PA1-031 was used in Western Blotting to investigate the network of genes that controls miRNA transcription and processing during Caenorhabditis elegans ageing.

Thu Feb 01 00:00:00 EST 2018

A novel function for the DEAD-box RNA helicase DDX-23 in primary microRNA processing in Caenorhabditis elegans.

Developmental biology

Chu YD,Chen HK,Huang T,Chan SP

PA1-031X was used in western blot to identify DEAD/H-box proteins involved in miRNA biogenesis

Fri Jan 15 00:00:00 EST 2016

Identifying Argonaute binding sites in Caenorhabditis elegans using iCLIP.

Methods (San Diego, Calif.)

Broughton JP,Pasquinelli AE

PA1-031 was used in Western Blotting to identify Argonaute binding sites in Caenorhabditis elegans using individual nucleotide resolution crosslinking and immunoprecipitation.

Sun Sep 15 00:00:00 EDT 2013

Identifying Argonaute binding sites in Caenorhabditis elegans using iCLIP.

Methods (San Diego, Calif.)

Broughton JP,Pasquinelli AE

PA1-031 was used in Western Blotting to identify Argonaute binding sites in Caenorhabditis elegans using individual nucleotide resolution crosslinking and immunoprecipitation.