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Bethyl Laboratories
ASH2 Polyclonal Antibody
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Product Details
A300-112A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 225 and 275 of human Absent, Small, or Homeotic-Like 2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Its predicted molecular weight is 68.7kDa. Alternative splicing results in possible isoforms at 60 kDa & 56.5 kDa). Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. Homology to mouse ASH2L is 90%. ASH2L is predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes as well as leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cell lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. In addition ASH2L has been suggested to interact with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASH 2; ash2 (absent, small, or homeotic)-like; ASH2 LIKE; ASH2 like protein; ASH2-like protein; ASH2L; Bre 2; Set1/Ash2 histone methyltransferase complex subunit ASH2; unnamed protein product
Gene Aliases: ASH2; ASH2L; ASH2L1; ASH2L2; Bre2
UniProt ID: (Human) Q9UBL3, (Mouse) Q91X20
Entrez Gene ID: (Human) 9070, (Mouse) 23808
transcription regulatory region sequence-specific DNA binding
DNA binding
protein binding
beta-catenin binding
zinc ion binding
metal ion binding
histone methyltransferase activity (H3-K4 specific)
transcription regulatory region DNA binding
euchromatin binding
chromatin/chromatin-binding, or -regulatory protein
histone modifying enzyme
chromatin organization
cellular response to DNA damage stimulus
positive regulation of cell proliferation
regulation of gene expression
positive regulation of macromolecule biosynthetic process
hemopoiesis
response to estrogen
positive regulation of gene expression, epigenetic
transcription, DNA-templated
regulation of transcription, DNA-templated
chromatin modification
positive regulation of transcription from RNA polymerase II promoter
chromatin-mediated maintenance of transcription
histone H3-K4 methylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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