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ATP1A2 Polyclonal Antibody

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ATP1A2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using ATP1A2 antibody 16836-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

ATP1A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 16836-1-AP (ATP1A2 Antibody) at dilution of 1:200 (under 40x lens).

ATP1A2 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 16836-1-AP (ATP1A2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

ATP1A2 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.5ug ATP1A2 antibody (16836-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IGG with dilution 1:100.

Product Details

16836-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Flow Cytometry (Flow)	0.5 µg/1x10^6 cells	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Dog, Human, Mollusc, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATP1A2 Fusion Protein Ag10515 (347-700 aa encoded by BC052271) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody was raised against the internal region of the human ATP1A2 and can recognize all the isoforms of alpha subunit. The 65 kDa band detected occasionally may be the degradation product of ATP1A2.

Immunogen sequence: KRMARKNCL VKNLEAVETL GSTSTICSDK TGTLTQNRMT VAHMWFDNQI HEADTTEDQS GATFDKRSPT WTALSRIAGL CNRAVFKAGQ ENISVSKRDT AGDASESALL KCIELSCGSV RKMRDRNPKV AEIPFNSTNK YQLSIHERED SPQSHVLVMK GAPERILDRC STILVQGKEI PLDKEMQDAF QNAYMELGGL GERVLGFCQL NLPSGKFPRG FKFDTDELNF PTEKLCFVGL MSMIDPPRAA VPDAVGKCRS AGIKVIMVTG DHPITAKAIA KGVGIISEGN ETVEDIAARL NIPMSQVNPR EAKACVVHGS DLKDMTSEQL DEILKNHTEI VFARTSPQQK LIIVE (347-700 aa encoded by BC052271)

Target Information

Atp1a2 encodes an Na+/K+ ATPase, which is a membrane protein that is composed of two subunits - alpha and beta. The Na+/K+ ATPase pump maintains an essential electrochemical gradient within cells through the active transport of sodium (Na+) and potassium (K+) ions. The Atp1a2 encoded protein belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These ion gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. The ATPase enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta), and the catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. The Atp1a2 gene encodes an alpha 2 subunit. Mutations in the Atp1a2 gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. Diseases associated with ATP1A2 include Alternating Hemiplegia Of Childhood and Migraine, Familial Hemiplegic, 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression of Na⁺/K⁺-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai.

Frontiers in physiology

Jia Y,Liu X

Thu Oct 01 00:00:00 EDT 2020

Evaluating the involvement of cerebral microvascular endothelial Na⁺/K⁺-ATPase and Na⁺-K⁺-2Cl^- co-transporter in electrolyte fluxes in an in vitro blood-brain barrier model of dehydration.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Lykke K,Assentoft M,Hørlyck S,Helms HC,Stoica A,Toft-Bertelsen TL,Tritsaris K,Vilhardt F,Brodin B,MacAulay N

Fri Mar 01 00:00:00 EST 2019

Role of RAB5A in FSHR-mediated signal transduction in human granulosa cells.

Reproduction (Cambridge, England)

Zhu K,Li S,Liu J,Hong Y,Chen ZJ,Du Y

Fri Jun 01 00:00:00 EDT 2018

The α2β2 isoform combination dominates the astrocytic Na⁺ /K⁺ -ATPase activity and is rendered nonfunctional by the α2.G301R familial hemiplegic migraine type 2-associated mutation.

Glia

Stoica A,Larsen BR,Assentoft M,Holm R,Holt LM,Vilhardt F,Vilsen B,Lykke-Hartmann K,Olsen ML,MacAulay N

Wed Nov 01 00:00:00 EDT 2017

A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study.

PloS one

Liu X,Shen Y,Xie J,Bao H,Cao Q,Wan R,Xu X,Zhou H,Huang L,Xu Z,Zhu W,Hu J,Cheng X,Hong K

Mon Sep 11 00:00:00 EDT 2017

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Molecular biology of the cell

Plössl K,Royer M,Bernklau S,Tavraz NN,Friedrich T,Wild J,Weber BHF,Friedrich U

Tue Aug 01 00:00:00 EDT 2017

Elimination of undifferentiated human embryonic stem cells by cardiac glycosides.

Scientific reports

Lin YT,Wang CK,Yang SC,Hsu SC,Lin H,Chang FP,Kuo TC,Shen CN,Chiang PM,Hsiao M,Lu FL,Lu J

Thu Jul 13 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: ATPase Na+/K+ transporting alpha 2 polypeptide; ATPase, Na+/K+ transporting, alpha 2 polypeptide; ATPase, Na+K+ transporting, alpha 2; Na(+)/K(+) ATPase alpha(+) subunit; Na(+)/K(+) ATPase alpha-2 subunit; Na+/K+ -ATPase alpha 2 subunit; Na+/K+ ATPase, alpha-A(+) catalytic polypeptide; Na+/K+ ATPase, alpha-B polypeptide; Sodium Potassium ATPase; Sodium pump subunit alpha-2; sodium-potassium ATPase catalytic subunit alpha-2; sodium/potassium-transporting ATPase alpha-2 chain; Sodium/potassium-transporting ATPase subunit alpha-2

Gene Aliases: ATP1A2; Atpa-3; AW060654; FHM2; KIAA0778; MHP2; mKIAA0778; RATATPA2

UniProt ID: (Human) P50993, (Rat) P06686, (Mouse) Q6PIE5

Entrez Gene ID: (Human) 477, (Rat) 24212, (Mouse) 98660

Molecular Function: primary active transporter