Search
Invitrogen
ATRX Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
ATRX Antibody (PA5-104497) in ICC/IF
Immunocytochemistry analysis of ATRX in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ATRX polyclonal antibody (Product # PA5-104497) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104497
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATRX(Accession P46100), corresponding to amino acid residues T125-G175.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ATRX.
Target Information
ATRX encodes a protein that contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: alpha thalassemia/mental retardation syndrome (X-linked); ATP-dependent helicase ATRX; ATRX; helicase 2; Heterochromatin protein 2; HP1 alpha-interacting protein; HP1-BP38 protein; RP5-875J14.1; Transcriptional regulator ATRX; unnamed protein product; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX
Gene Aliases: 4833408C14Rik; AI447451; ATR2; ATRX; DXHXS6677E; HP1-BP38; Hp1bp2; Hp1bp38; JMS; MRX52; MRXS3; RAD54; RAD54L; XH2; XNP; ZNF-HX
UniProt ID: (Human) P46100, (Mouse) Q61687
Entrez Gene ID: (Human) 546, (Mouse) 22589
nucleotide binding
DNA binding
chromatin binding
helicase activity
protein binding
ATP binding
zinc ion binding
DNA translocase activity
hydrolase activity
ATPase activity
chromatin DNA binding
histone binding
metal ion binding
histone H3K9me2/3 reader activity
chromo shadow domain binding
methylated histone binding
DNA-binding transcription factor
gene-specific transcriptional regulator
meiotic spindle organization
DNA repair
chromatin organization
nucleosome assembly
chromatin remodeling
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
spermatogenesis
regulation of cell cycle process
positive regulation of nuclear cell cycle DNA replication
DNA damage response, signal transduction by p53 class mediator
forebrain development
replication fork processing
telomeric heterochromatin assembly
positive regulation of telomere maintenance
regulation of chromosome organization
post-embryonic forelimb morphogenesis
multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
Sertoli cell development
chromosome organization involved in meiotic cell cycle
protein localization to chromosome, telomeric region
seminiferous tubule development
cellular response to hydroxyurea
negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric
DNA replication-independent nucleosome assembly
transcription, DNA-templated
chromatin modification
negative regulation of telomeric RNA transcription from RNA pol II promoter
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support