Invitrogen

BMPR2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (17) BMPR2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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BMPR2 Antibody in Western Blot (WB) — Western blot was performed using Anti-BMPR2 Polyclonal Antibody (Product # PA5-21437) and a 115 kDa band corresponding to BMPR2 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HUV-EC-C (Lane 1), U-2 OS (Lane 2), tissue extracts (30 µg lysate) of Mouse Brain (Lane 3) and Rat Brain (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

BMPR2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of BMPR2 was observed in Mouse Adipose when compared to Mouse Esophagus using BMPR2 Polyclonal Antibody (Product # PA5-21437) in western blot.

Product Details

PA5-21437

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 667 and 951 of BMPR2 (Uniprot ID#Q13873)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152912

Product Specific Information

Recommended positive controls: HUVEC.

Predicted reactivity: Mouse (92%), Rat (92%), Pig (92%), Chicken (86%), Sheep (93%), Rhesus Monkey (98%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BMP type II receptor; BMP type-2 receptor; BMPR-2; Bone morphogenetic protein receptor type II; Bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase); bone morphogenic protein receptor, type II (serine/threonine kinase); BRK-3; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4

Gene Aliases: 2610024H22Rik; AL117858; AW546137; BB189135; BMP-2; BMPR-2; BMPR-II; BMPR2; BMPR3; BMPRII; BMR2; BRK-3; Gm20272; POVD1; PPH1; T-ALK

UniProt ID: (Human) Q13873, (Mouse) O35607

Entrez Gene ID: (Human) 659, (Rat) 140590, (Mouse) 12168

Molecular Function: serine/threonine protein kinase receptor