Proteintech

CCP1 Polyclonal Antibody

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CCP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed A549 cells using 14067-1-AP (CCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CCP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 14067-1-AP ( CCP1 Antibody) at dilution of 1:200 (under 10x lens).

CCP1 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CCP1 Antibody in Immunoprecipitation (IP) — IP result of anti-CCP1 (IP:14067-1-AP, 3ug; Detection:14067-1-AP 1:1000) with mouse testis tissue lysate 10000ug.

Product Details

14067-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CCP1 Fusion Protein Ag5122 (837-1186 aa encoded by BC060815) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: VLCETLSGN SCPLVTITAM PESNYYEHIC HFRNRPYVFL SARVHPGETN ASWVMKGTLE YLMSNNPTAQ SLRESYIFKI VPMLNPDGVI NGNHRCSLSG EDLNRQWQSP SPDLHPTIYH AKGLLQYLAA VKRLPLVYCD YHGHSRKKNV FMYGCSIKET VWHTNDNATS CDVVEDTGYR TLPKILSHIA PAFCMSSCSF VVEKSKESTA RVVVWREIGV QRSYTMESTL CGCDQGKYKG LQIGTRELEE MGAKFCVGLL RLKRLTSPLE YNLPSSLLDF ENDLIESSCK VTSPTTYVLD EDEPRFLEEV DYSAESNDEL DIELAENVGD YEPSAQEEVL SDSELSRTYL P (837-1186 aa encoded by BC060815)

Target Information

NNA1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motif that was initially cloned from regenerating spinal cord neurons of the mouse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice.

The Journal of cell biology

Gilmore-Hall S,Kuo J,Ward JM,Zahra R,Morrison RS,Perkins G,La Spada AR

Mon Jan 07 00:00:00 EST 2019

CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice.

The Journal of cell biology

Gilmore-Hall S,Kuo J,Ward JM,Zahra R,Morrison RS,Perkins G,La Spada AR

Mon Jan 07 00:00:00 EST 2019

Loss of tubulin deglutamylase CCP1 causes infantile-onset neurodegeneration.

The EMBO journal

Shashi V,Magiera MM,Klein D,Zaki M,Schoch K,Rudnik-Schöneborn S,Norman A,Lopes Abath Neto O,Dusl M,Yuan X,Bartesaghi L,De Marco P,Alfares AA,Marom R,Arold ST,Guzmán-Vega FJ,Pena LD,Smith EC,Steinlin M,Babiker MO,Mohassel P,Foley AR,Donkervoort S,Kaur R,Ghosh PS,Stanley V,Musaev D,Nava C,Mignot C,Keren B,Scala M,Tassano E,Picco P,Doneda P,Fiorillo C,Issa MY,Alassiri A,Alahmad A,Gerard A,Liu P,Yang Y,Ertl-Wagner B,Kranz PG,Wentzensen IM,Stucka R,Stong N,Allen AS,Goldstein DB,Schoser B,Rösler KM,Alfadhel M,Capra V,Chrast R,Strom TM,Kamsteeg EJ,Bönnemann CG,Gleeson JG,Martini R,Janke C,Senderek J

Mon Dec 03 00:00:00 EST 2018

Loss of tubulin deglutamylase CCP1 causes infantile-onset neurodegeneration.

The EMBO journal

Mon Dec 03 00:00:00 EST 2018

Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.

Nature communications

Ye B,Liu B,Hao L,Zhu X,Yang L,Wang S,Xia P,Du Y,Meng S,Huang G,Qin X,Wang Y,Yan X,Li C,Hao J,Zhu P,He L,Tian Y,Fan Z

Wed Mar 28 00:00:00 EDT 2018

Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis.

Cell

Silva CG,Peyre E,Adhikari MH,Tielens S,Tanco S,Van Damme P,Magno L,Krusy N,Agirman G,Magiera MM,Kessaris N,Malgrange B,Andrieux A,Janke C,Nguyen L

Thu Feb 22 00:00:00 EST 2018

Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer's disease.

Scientific reports

Vu HT,Akatsu H,Hashizume Y,Setou M,Ikegami K

Mon Jan 09 00:00:00 EST 2017

Bioinformatics Data Mining Approach Suggests Coexpression of AGTPBP1 with an ALS-linked Gene C9orf72.

Journal of central nervous system disease

Kitano S,Kino Y,Yamamoto Y,Takitani M,Miyoshi J,Ishida T,Saito Y,Arima K,Satoh J

Wed Jun 24 00:00:00 EDT 2015

Bioinformatics Data Mining Approach Suggests Coexpression of AGTPBP1 with an ALS-linked Gene C9orf72.

Journal of central nervous system disease

Kitano S,Kino Y,Yamamoto Y,Takitani M,Miyoshi J,Ishida T,Saito Y,Arima K,Satoh J

Wed Jun 24 00:00:00 EDT 2015

AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.

Nature communications

Herms A,Bosch M,Reddy BJ,Schieber NL,Fajardo A,Rupérez C,Fernández-Vidal A,Ferguson C,Rentero C,Tebar F,Enrich C,Parton RG,Gross SP,Pol A

Wed May 27 00:00:00 EDT 2015

Functional segregation and emerging role of cilia-related cytosolic carboxypeptidases (CCPs).

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Rodríguez de la Vega Otazo M,Lorenzo J,Tort O,Avilés FX,Bautista JM

Fri Feb 01 00:00:00 EST 2013

Cytosolic carboxypeptidase 1 is involved in processing α- and β-tubulin.

The Journal of biological chemistry

Berezniuk I,Vu HT,Lyons PJ,Sironi JJ,Xiao H,Burd B,Setou M,Angeletti RH,Ikegami K,Fricker LD

Fri Feb 24 00:00:00 EST 2012

Abnormal sperm development in pcd(3J)-/- mice: the importance of Agtpbp1 in spermatogenesis.

Molecules and cells

Kim N,Xiao R,Choi H,Jo H,Kim JH,Uhm SJ,Park C

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: ATP/GTP-binding protein 1; carboxypeptidase-tubulin; Cytosolic carboxypeptidase 1; DKFZp686M20191; Nervous system nuclear protein induced by axotomy protein 1; Nervous system nuclear protein induced by axotomy protein 1 homolog; nuclear ATP/GTP-binding protein; Purkinje cell degeneration; soluble carboxypeptidase; tubulinyl-Tyr carboxypeptidase; tyrosine carboxypeptidase

Gene Aliases: 1700020N17Rik; 2310001G17Rik; 2900054O13Rik; 4930445M19Rik; 5730402G09Rik; AGTPBP1; CCP1; KIAA1035; nmf243; NNA1; pcd

UniProt ID: (Human) Q9UPW5, (Mouse) Q641K1

Entrez Gene ID: (Human) 23287, (Mouse) 67269

Molecular Function: metalloprotease