Bethyl Laboratories

CDK5RAP3 Polyclonal Antibody

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CDK5RAP3 Antibody in Western Blot (WB) — **Detection of human CDK5RAP3 by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-CDK5RAP3 antibody BL3564 (Cat. No. A300-871A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). CDK5RAP3 was also immunoprecipitated by rabbit anti-CDK5RAP3 antibody BL3563 (Cat. No. A300-870A), which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Product Details

A300-871A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-
Immunoprecipitation (IP)	2-5 µg/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 450 and C-term View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	tris citrate/phosphate, pH 7-8
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan

Target Information

This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein IC53-2; CDK5 regulatory subunit-associated protein 3; IC53-2; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARF-binding protein; LXXLL/leucine-zipper-containing ARFbinding protein; Protein HSF-27

Gene Aliases: C53; CDK5RAP3; HSF-27; IC53; LZAP; MST016; MSTP016; OK/SW-cl.114; PP1553

UniProt ID: (Human) Q96JB5

Entrez Gene ID: (Human) 80279

Function(s)