Bethyl Laboratories

CDK5RAP3 Polyclonal Antibody

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CDK5RAP3 Antibody in Western Blot (WB) — **Detection of human CDK5RAP3 by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-CDK5RAP3 antibody BL3564 (Cat. No. A300-871A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). CDK5RAP3 was also immunoprecipitated by rabbit anti-CDK5RAP3 antibody BL3563 (Cat. No. A300-870A), which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Product Details

Tested Applications

Dilution

Immunoprecipitation (IP)

2-5 µg/mg lysate

Western Blot (WB)

1:2,000-1:10,000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 450 and C-term

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan

Target Information

This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein IC53-2; CDK5 regulatory subunit-associated protein 3; IC53-2; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARF-binding protein; LXXLL/leucine-zipper-containing ARFbinding protein; Protein HSF-27

Gene Aliases: C53; CDK5RAP3; HSF-27; IC53; LZAP; MST016; MSTP016; OK/SW-cl.114; PP1553

UniProt ID: (Human) Q96JB5

Entrez Gene ID: (Human) 80279

Function(s)

regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of protein phosphorylation mitotic G2 DNA damage checkpoint brain development cell proliferation regulation of phosphatase activity apoptotic nuclear changes endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity negative regulation of MAP kinase activity negative regulation of protein kinase activity by regulation of protein phosphorylation mitotic G2/M transition checkpoint regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter protein ufmylation negative regulation of protein serine/threonine kinase activity positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator negative regulation of cellular protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process

Process(es)

protein binding protein kinase binding cyclin binding protein complex binding ubiquitin-like protein ligase binding mitogen-activated protein kinase binding NF-kappaB binding MDM2/MDM4 family protein binding

CDK5RAP3 Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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