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Agrisera
CGL78/YCF54 Polyclonal Antibody
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FIGURE: 1 / 1
CGL78/YCF54 Antibody (AS10-936) in WB
15 µg of total protein from Arabidopsis thaliana (ecotype Col-0), Chlamydomonas reinhardtii (strain 4 A+) and Synechocystis sp. (strain PCC 6803 / Kazusa), extracted with 56 m M Na 2 CO 3,56 m M DTT, 1% (w/v) SDS, 12% (w/v) Sucrose, 2 m M EDTA) were separated on 15% SDS-PAGE and blotted 1 h to nitrocellulose membrane. Blot was blocked with 2% milk powder in TBS-T for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 1 h at RT with agitation. The antibody solution was decant... View More
Product Details
AS10-936
Product Specifications
Species Reactivity
Algae,
Bacteria,
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment of Arabidopsis thaliana CGL78.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Synechocystis sp. PCC 6803
Target Information
CGL78 encodes a small chloroplast-localized auxiliary factor (the algal homolog of Ycf54) that is associated with thylakoid/pigment biogenesis and is required for normal chlorophyll accumulation. In Chlamydomonas reinhardtii, CGL78 functions within the chlorophyll biosynthetic pathway at the oxygen-dependent Mg-protoporphyrin IX monomethyl ester cyclase step that forms the isocyclic E ring en route to protochlorophyllide; consistent with this role, reducing CGL78 expression causes chlorosis, decreases total chlorophyll, and destabilizes photosystem I-light-harvesting complex I supercomplexes with reduced accumulation of PSI subunits (including PSAK). Across oxygenic phototrophs, Ycf54/CGL78 is viewed as an auxiliary component that supports cyclase activity (e.g., by promoting the level and/or activity of the catalytic AcsF/CycI subunit), thereby coupling tetrapyrrole cofactor production to photosystem assembly and photosynthetic competence, particularly under stress conditions such as copper deficiency where CGL78 is induced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ycf54
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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