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Invitrogen
CHP2 Polyclonal Antibody
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FIGURE: 1 / 2
CHP2 Antibody (PA5-101475) in IHC (P)
Immunohistochemistry analysis of CHP2 in human liver cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CHP2 polyclonal antibody (Product # PA5-101475) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-101475
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CHP2(Accession O43745), corresponding to amino acid residues P103-L153.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CHP2.
Target Information
CHP2 (Calcineurin B homologous protein 2), also known as Hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains four EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcineurin B homologous protein 2; Chp-2; hepatocellular carcinoma antigen gene 520; Hepatocellular carcinoma-associated antigen 520; Hepatocellular carcinoma-associated antigen 520 homolog; LCA520; unnamed protein product
Gene Aliases: 2010110P09Rik; Cbhp2; chp-2; CHP2; HCA520
UniProt ID: (Human) O43745, (Rat) Q810D1, (Mouse) Q9D869
Entrez Gene ID: (Human) 63928, (Rat) 308965, (Mouse) 70261
cellular sodium ion homeostasis
positive regulation of cell proliferation
positive regulation of phosphatase activity
protein transport
regulation of cellular pH
positive regulation of protein import into nucleus
positive regulation of transcription from RNA polymerase II promoter
positive regulation of calcineurin-NFAT signaling cascade
cellular response to calcium ion
regulation of protein import into nucleus
regulation of calcineurin-NFAT signaling cascade
transport
sodium ion transport
regulation of pH
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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