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Invitrogen
CYP2C11 Polyclonal Antibody
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FIGURE: 1 / 6
CYP2C11 Antibody (PA3-034) in ICC/IF
Immunofluorescent analysis of Cytochrome P450 2C11 (green) showing staining in the membrane of H-4-II-E cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 2C11 polyclonal antibody (Product # PA3-034) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room t... View More
Product Details
PA3-034
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat,
Human,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-034 detects cytochrome P450 2C11 (P450 2C11) from human, feline and rat tissues.
PA3-034 has been successfully used in Western blot, ICC/IF and immunohistochemical (frozen and paraffin) procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 2C11 from rat liver extract. Immunohistochemical staining of P450 2C11 in rat brain with PA3-034 results in perivascular staining.
The PA3-034 immunogen is a synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11.
Target Information
The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA's which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EET's, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EET's are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EET's are converted from AA by the 2C11 family of P450's whose expression is induced by testosterone and is therefore not generally found in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CYP; CYPIIC11; Cytochrome P-450(M-1); Cytochrome P450 2C11; cytochrome P450, 2c29; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase); Cytochrome P450-UT-2; Cytochrome P450-UT-A; Cytochrome P450H; P-450(M-1); P450; P450-UT-A; P450H; UT-2
Gene Aliases: Cyp2c; Cyp2c-11; Cyp2c11; CYP2CII
UniProt ID: (Rat) P08683
Entrez Gene ID: (Rat) 29277
iron ion binding
testosterone 16-alpha-hydroxylase activity
arachidonic acid epoxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
heme binding
aromatase activity
oxidoreductase
oxygenase
metabolite interconversion enzyme
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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