PA3-034 detects cytochrome P450 2C11 (P450 2C11) from human, feline and rat tissues.
PA3-034 has been successfully used in Western blot, ICC/IF and immunohistochemical (frozen and paraffin) procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 2C11 from rat liver extract. Immunohistochemical staining of P450 2C11 in rat brain with PA3-034 results in perivascular staining.
The PA3-034 immunogen is a synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11.
The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA's which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EET's, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EET's are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EET's are converted from AA by the 2C11 family of P450's whose expression is induced by testosterone and is therefore not generally found in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CYP; CYPIIC11; Cytochrome P-450(M-1); Cytochrome P450 2C11; cytochrome P450, 2c29; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase); Cytochrome P450-UT-2; Cytochrome P450-UT-A; Cytochrome P450H; P-450(M-1); P450; P450-UT-A; P450H; UT-2
Gene Aliases: Cyp2c; Cyp2c-11; Cyp2c11; CYP2CII
UniProt ID: (Rat) P08683
Entrez Gene ID: (Rat) 29277