|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Full length human CDC42|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28295 detects CDC42 from human, mouse, rat samples.
PA1-28295 has been successfully used in Western blot applications.
The PA1-28295 immunogen is: Full length human CDC42
This protein is a small GTPase of the Rho subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis, and cell progrssion. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42 1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrick syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in at least two transcipt variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.