Description: This LP3K monoclonal antibody reacts with human cytokeratin 8 (K8), a 55-kDa member of the family of intermediate filament proteins. Cytokeratin 8 is a type II (or basic) keratin that is expressed in epithelial and carcinoma cells. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. More specifically, studies have demonstrated the involvement of cytokeratin 8 in protection against apoptosis, stress, or injury, as well as regulation of the cell cycle. This keratin is frequently co-expressed with cytokeratin 18, a type I (or acidic) keratin as a heterodimer. Although detected primarily in the cytoplasm of normal healthy cells, cytokeratin 8 has been found to localize to the plasma membrane in some tumor cells. Finally, cytokeratin 8 is phosphorylated on serine 73 in dividing cells.
Applications Reported: This LP3K antibody has been reported for use in immunoblotting (WB) and immunocytochemistry.
Applications Tested: This LP3K antibody has been tested by western blot analysis of reduced A549 cell lysate, as well as by immunocytochemistry of A549 cells. For western blotting, this antibody can be used at 1-10 µg/mL. For immunocytochemistry, LP3K can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CK-8; Cytokeratin-8; K-8; K8; Keratin; keratin 8, type II; Keratin, type II cytoskeletal 8; Keratin-8; type II cytoskeletal 8; Type-II keratin Kb8
Gene Aliases: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; KRT8
UniProt ID: (Human) P05787
Entrez Gene ID: (Human) 3856