Proteintech

DAAM1 Polyclonal Antibody

View all (7) DAAM1 antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

DAAM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using DAAM1 antibody 14876-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DAAM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 14876-1-AP (DAAM1 antibody) at dilution of 1:100 (under 40x lens).

DAAM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 14876-1-AP (DAAM1 antibody) at dilution of 1:100 (under 10x lens).

DAAM1 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DAAM1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DAAM1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DAAM1 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DAAM1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DAAM1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

DAAM1 Antibody in Immunoprecipitation (IP) — IP result of anti-DAAM1 (IP:14876-1-AP, 3ug; Detection:14876-1-AP 1:500) with mouse liver tissue lysate 8000ug.

Product Details

14876-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DAAM1 Fusion Protein Ag6648 (715-1068 aa encoded by BC064999) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.

Immunogen sequence: KDMLEQLLK FVPEKSDIDL LEEHKHELDR MAKADRFLFE MSRINHYQQR LQSLYFKKKF AERVAEVKPK VEAIRSGSEE VFRSGALKQL LEVVLAFGNY MNKGQRGNAY GFKISSLNKI ADTKSSIDKN ITLLHYLITI VENKYPSVLN LNEELRDIPQ AAKVNMTELD KEISTLRSGL KAVETELEYQ KSQPPQPGDK FVSVVSQFIT VASFSFSDVE DLLAEAKDLF TKAVKHFGEE AGKIQPDEFF GIFDQFLQAV SEAKQENENM RKKKEEEERR ARMEAQLKEQ RERERKMRKA KENSEESGEF DDLVSALRSG EVFDKDLSKL KRNRKRITNQ MTDSSRERPI TKLNF (715-1068 aa encoded by BC064999)

Target Information

DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1.

PloS one

Williams SK,Weiner ZP,Gilmore RD

Tue Dec 11 00:00:00 EST 2018

Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1.

PloS one

Williams SK,Weiner ZP,Gilmore RD

Tue Dec 11 00:00:00 EST 2018

Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1.

PloS one

Williams SK,Weiner ZP,Gilmore RD

Tue Dec 11 00:00:00 EST 2018

Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Molecular biology of the cell

Rana MK,Aloisio FM,Choi C,Barber DL

Fri Jun 15 00:00:00 EDT 2018

miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer.

Cellular signalling

Xiong H,Yan T,Zhang W,Shi F,Jiang X,Wang X,Li S,Chen Y,Chen C,Zhu Y

Sun Apr 01 00:00:00 EDT 2018

Implication of overexpression of dishevelled-associated activator of morphogenesis 1 (Daam-1) for the pathogenesis of human Idiopathic Pulmonary Arterial Hypertension (IPAH).

Diagnostic pathology

Yanai S,Wakayama M,Nakayama H,Shinozaki M,Tsukuma H,Tochigi N,Nemoto T,Saji T,Shibuya K

Tue Mar 14 00:00:00 EDT 2017

DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility.

The Journal of cell biology

Nishimura T,Ito S,Saito H,Hiver S,Shigetomi K,Ikenouchi J,Takeichi M

Mon Nov 21 00:00:00 EST 2016

DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility.

The Journal of cell biology

Nishimura T,Ito S,Saito H,Hiver S,Shigetomi K,Ikenouchi J,Takeichi M

Mon Nov 21 00:00:00 EST 2016

Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis.

Development (Cambridge, England)

Li D,Hallett MA,Zhu W,Rubart M,Liu Y,Yang Z,Chen H,Haneline LS,Chan RJ,Schwartz RJ,Field LJ,Atkinson SJ,Shou W

Sat Jan 01 00:00:00 EST 2011

Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis.

Development (Cambridge, England)

Li D,Hallett MA,Zhu W,Rubart M,Liu Y,Yang Z,Chen H,Haneline LS,Chan RJ,Schwartz RJ,Field LJ,Atkinson SJ,Shou W

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: Disheveled-associated activator of morphogenesis 1

Gene Aliases: DAAM1; KIAA0666

UniProt ID: (Human) Q9Y4D1, (Mouse) Q8BPM0

Entrez Gene ID: (Human) 23002, (Mouse) 208846, (Rat) 314212

cellular component organization actin cytoskeleton organization Wnt signaling pathway, planar cell polarity pathway

Process(es)

actin binding protein binding Rho GTPase binding identical protein binding