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Product Details
14876-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
DAAM1 Fusion Protein Ag6648 (715-1068 aa encoded by BC064999)
Conjugate
Form
Liquid
Concentration
0.21 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.
Immunogen sequence: KDMLEQLLK FVPEKSDIDL LEEHKHELDR MAKADRFLFE MSRINHYQQR LQSLYFKKKF AERVAEVKPK VEAIRSGSEE VFRSGALKQL LEVVLAFGNY MNKGQRGNAY GFKISSLNKI ADTKSSIDKN ITLLHYLITI VENKYPSVLN LNEELRDIPQ AAKVNMTELD KEISTLRSGL KAVETELEYQ KSQPPQPGDK FVSVVSQFIT VASFSFSDVE DLLAEAKDLF TKAVKHFGEE AGKIQPDEFF GIFDQFLQAV SEAKQENENM RKKKEEEERR ARMEAQLKEQ RERERKMRKA KENSEESGEF DDLVSALRSG EVFDKDLSKL KRNRKRITNQ MTDSSRERPI TKLNF (715-1068 aa encoded by BC064999)
Target Information
DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Disheveled-associated activator of morphogenesis 1
Gene Aliases: DAAM1; KIAA0666
UniProt ID: (Human) Q9Y4D1, (Mouse) Q8BPM0
Entrez Gene ID: (Human) 23002, (Mouse) 208846, (Rat) 314212
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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