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DACH1 Polyclonal Antibody

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DACH1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 10914-1-AP (DACH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 10914-1-AP ( DACH1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

DACH1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10914-1-AP ( DACH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

DACH1 Antibody in Immunoprecipitation (IP) — IP result of anti-DACH1 (IP:10914-1-AP, 4ug; Detection:10914-1-AP 1:1000) with HEK-293 cells lysate 6000ug.

Product Details

10914-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 16 publications 16 publications
Immunohistochemistry (IHC)	-	View 14 publications 14 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:200	View 20 publications 20 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-
ChIP assay (ChIP)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DACH1 Fusion Protein Ag1354 (176-521 aa encoded by BC021219) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.14 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

Immunogen sequence: QHGADSENG DMNSSVGLEL PFMMMPHPLI PVSLPPASVT MAMSQMNHLS TIANMAAAAQ VQSPPSRVET SVIKERVPDS PSPAPSLEEG RRPGSHPSSH RSSSVSSSPA RTESSSDRIP VHQNGLSMNQ MLMGLSPNVL PGPKEGDLAG HDMGHESKRM HIEKDETPLS TPTARDSLDK LSLTGHGQPL PPGFPSPFLF PDGLSSIETL LTNIQGLLKV AIDNARAQEK QVQLEKTELK MDFLRERELR ETLEKQLAME QKNRAIVQKR LKKEKKAKRK LQEALEFETK RREQAEQTLK QAASTDSLRV LNDSLTPEIE ADRSGGRTDA ERTIQDGRLY LKTTVMY (176-521 aa encoded by BC021219 )

Target Information

Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Metformin blocks myeloid-derived suppressor cell accumulation through AMPK-DACH1-CXCL1 axis.

Oncoimmunology

Qin G,Lian J,Huang L,Zhao Q,Liu S,Zhang Z,Chen X,Yue D,Li L,Li F,Wang L,Umansky V,Zhang B,Yang S,Zhang Y

Tue Jan 12 00:00:00 EST 2021

Metformin blocks myeloid-derived suppressor cell accumulation through AMPK-DACH1-CXCL1 axis.

Oncoimmunology

Qin G,Lian J,Huang L,Zhao Q,Liu S,Zhang Z,Chen X,Yue D,Li L,Li F,Wang L,Umansky V,Zhang B,Yang S,Zhang Y

Tue Jan 12 00:00:00 EST 2021

MicroRNA-6807-3p promotes the tumorigenesis of glioma by targeting downstream DACH1.

Brain research

Lu GF,Geng F,Xiao Z,Chen YS,Han Y,You CY,Gong NL,Xie ZM,Pan M

Mon Apr 01 00:00:00 EDT 2019

MicroRNA-6807-3p promotes the tumorigenesis of glioma by targeting downstream DACH1.

Brain research

Lu GF,Geng F,Xiao Z,Chen YS,Han Y,You CY,Gong NL,Xie ZM,Pan M

Mon Apr 01 00:00:00 EDT 2019

Global polysome analysis of normal and injured podocytes.

American journal of physiology. Renal physiology

Okabe M,Motojima M,Miyazaki Y,Pastan I,Yokoo T,Matsusaka T

Fri Feb 01 00:00:00 EST 2019

Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.

Stem cell reports

Jiao X,Li Z,Wang M,Katiyar S,Di Sante G,Farshchian M,South AP,Cocola C,Colombo D,Reinbold R,Zucchi I,Wu K,Tabas I,Spike BT,Pestell RG

Tue Jan 08 00:00:00 EST 2019

Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.

Stem cell reports

Jiao X,Li Z,Wang M,Katiyar S,Di Sante G,Farshchian M,South AP,Cocola C,Colombo D,Reinbold R,Zucchi I,Wu K,Tabas I,Spike BT,Pestell RG

Tue Jan 08 00:00:00 EST 2019

CCBE1 is required for coronary vessel development and proper coronary artery stem formation in the mouse heart.

Developmental dynamics : an official publication of the American Association of Anatomists

Bonet F,Pereira PNG,Bover O,Marques S,Inácio JM,Belo JA

Mon Oct 01 00:00:00 EDT 2018

Dachshund 1 is Differentially Expressed Between Male and Female Breast Cancer: A Matched Case-Control Study of Clinical Characteristics and Prognosis.

Clinical breast cancer

Cui Q,Kong D,Li Z,Ahiable P,Wang K,Wu K,Wu G

Mon Oct 01 00:00:00 EDT 2018

Modulation of inflammatory and hormonal parameters in response to testosterone therapy: Effects on the ventral prostate of adult rats.

Cell biology international

Mendes LO,Castilho ACS,Pinho CF,Gonçalvez BF,Razza EM,Chuffa LGA,Anselmo-Franci JA,Scarano WR,Martinez FE

Sat Sep 01 00:00:00 EDT 2018

Single-cell analysis of early progenitor cells that build coronary arteries.

Nature

Su T,Stanley G,Sinha R,D'Amato G,Das S,Rhee S,Chang AH,Poduri A,Raftrey B,Dinh TT,Roper WA,Li G,Quinn KE,Caron KM,Wu S,Miquerol L,Butcher EC,Weissman I,Quake S,Red-Horse K

Sun Jul 01 00:00:00 EDT 2018

Genome Editing in Neuroepithelial Stem Cells to Generate Human Neurons with High Adenosine-Releasing Capacity.

Stem cells translational medicine

Poppe D,Doerr J,Schneider M,Wilkens R,Steinbeck JA,Ladewig J,Tam A,Paschon DE,Gregory PD,Reik A,Müller CE,Koch P,Brüstle O

Fri Jun 01 00:00:00 EDT 2018

DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis.

Journal of hematology & oncology

Liu Q,Li A,Yu S,Qin S,Han N,Pestell RG,Han X,Wu K

Tue Apr 10 00:00:00 EDT 2018

DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis.

Journal of hematology & oncology

Liu Q,Li A,Yu S,Qin S,Han N,Pestell RG,Han X,Wu K

Tue Apr 10 00:00:00 EDT 2018

Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease.

Nature communications

Rhee S,Chung JI,King DA,D'amato G,Paik DT,Duan A,Chang A,Nagelberg D,Sharma B,Jeong Y,Diehn M,Wu JC,Morrison AJ,Red-Horse K

Thu Jan 25 00:00:00 EST 2018

CAMKIIγ suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis.

The Journal of clinical investigation

Doran AC,Ozcan L,Cai B,Zheng Z,Fredman G,Rymond CC,Dorweiler B,Sluimer JC,Hsieh J,Kuriakose G,Tall AR,Tabas I

Wed Nov 01 00:00:00 EDT 2017

Alternative Progenitor Cells Compensate to Rebuild the Coronary Vasculature in Elabela- and Apj-Deficient Hearts.

Developmental cell

Sharma B,Ho L,Ford GH,Chen HI,Goldstone AB,Woo YJ,Quertermous T,Reversade B,Red-Horse K

Mon Sep 25 00:00:00 EDT 2017

DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12-CXCR4 signaling axis.

Genes & development

Chang AH,Raftrey BC,D'Amato G,Surya VN,Poduri A,Chen HI,Goldstone AB,Woo J,Fuller GG,Dunn AR,Red-Horse K

Sat Jul 01 00:00:00 EDT 2017

DACH1 suppresses breast cancer as a negative regulator of CD44.

Scientific reports

Xu H,Yu S,Yuan X,Xiong J,Kuang D,Pestell RG,Wu K

Wed Jun 28 00:00:00 EDT 2017

Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.

Cell stem cell

Lorenz C,Lesimple P,Bukowiecki R,Zink A,Inak G,Mlody B,Singh M,Semtner M,Mah N,Auré K,Leong M,Zabiegalov O,Lyras EM,Pfiffer V,Fauler B,Eichhorst J,Wiesner B,Huebner N,Priller J,Mielke T,Meierhofer D,Izsvák Z,Meier JC,Bouillaud F,Adjaye J,Schuelke M,Wanker EE,Lombès A,Prigione A

Thu May 04 00:00:00 EDT 2017

Breathing control center neurons that promote arousal in mice.

Science (New York, N.Y.)

Yackle K,Schwarz LA,Kam K,Sorokin JM,Huguenard JR,Feldman JL,Luo L,Krasnow MA

Fri Mar 31 00:00:00 EDT 2017

Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions.

Neuroscience research

Isoda M,Kohyama J,Iwanami A,Sanosaka T,Sugai K,Yamaguchi R,Matsumoto T,Nakamura M,Okano H

Thu Sep 01 00:00:00 EDT 2016

YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression.

Nucleic acids research

Lyons SM,Achorn C,Kedersha NL,Anderson PJ,Ivanov P

Fri Aug 19 00:00:00 EDT 2016

Inhibition of DACH1 activity by short hairpin RNA represses cell proliferation and tumor invasion in pancreatic cancer.

Oncology reports

Bu XN,Qiu C,Wang C,Jiang Z

Mon Aug 01 00:00:00 EDT 2016

Partial Dedifferentiation of Murine Radial Glia-Type Neural Stem Cells by Brn2 and c-Myc Yields Early Neuroepithelial Progenitors.

Journal of molecular biology

Bung R,Wörsdörfer P,Thier MC,Lemke K,Gebhardt M,Edenhofer F

Sun Apr 10 00:00:00 EDT 2016

Specific Inhibition of β-Secretase Processing of the Alzheimer Disease Amyloid Precursor Protein.

Cell reports

Ben Halima S,Mishra S,Raja KMP,Willem M,Baici A,Simons K,Brüstle O,Koch P,Haass C,Caflisch A,Rajendran L

Tue Mar 08 00:00:00 EST 2016

Silencing DACH1 promotes esophageal cancer growth by inhibiting TGF-β signaling.

PloS one

Wu L,Herman JG,Brock MV,Wu K,Mao G,Yan W,Nie Y,Liang H,Zhan Q,Li W,Guo M

Fri Jun 05 00:00:00 EDT 2015

Silencing DACH1 promotes esophageal cancer growth by inhibiting TGF-β signaling.

PloS one

Wu L,Herman JG,Brock MV,Wu K,Mao G,Yan W,Nie Y,Liang H,Zhan Q,Li W,Guo M

Fri Jun 05 00:00:00 EDT 2015

The endogenous cell-fate factor dachshund restrains prostate epithelial cell migration via repression of cytokine secretion via a cxcl signaling module.

Cancer research

Chen K,Wu K,Jiao X,Wang L,Ju X,Wang M,Di Sante G,Xu S,Wang Q,Li K,Sun X,Xu C,Li Z,Casimiro MC,Ertel A,Addya S,McCue PA,Lisanti MP,Wang C,Davis RJ,Mardon G,Pestell RG

Fri May 15 00:00:00 EDT 2015

The endogenous cell-fate factor dachshund restrains prostate epithelial cell migration via repression of cytokine secretion via a cxcl signaling module.

Cancer research

Chen K,Wu K,Jiao X,Wang L,Ju X,Wang M,Di Sante G,Xu S,Wang Q,Li K,Sun X,Xu C,Li Z,Casimiro MC,Ertel A,Addya S,McCue PA,Lisanti MP,Wang C,Davis RJ,Mardon G,Pestell RG

Fri May 15 00:00:00 EDT 2015

APP processing in human pluripotent stem cell-derived neurons is resistant to NSAID-based γ-secretase modulation.

Stem cell reports

Mertens J,Stüber K,Wunderlich P,Ladewig J,Kesavan JC,Vandenberghe R,Vandenbulcke M,van Damme P,Walter J,Brüstle O,Koch P

Mon May 11 00:00:00 EDT 2015

Estrogen receptor β exon 3-deleted mouse: The importance of non-ERE pathways in ERβ signaling.

Proceedings of the National Academy of Sciences of the United States of America

Maneix L,Antonson P,Humire P,Rochel-Maia S,Castañeda J,Omoto Y,Kim HJ,Warner M,Gustafsson JÅ

Tue Apr 21 00:00:00 EDT 2015

Estrogen receptor β exon 3-deleted mouse: The importance of non-ERE pathways in ERβ signaling.

Proceedings of the National Academy of Sciences of the United States of America

Maneix L,Antonson P,Humire P,Rochel-Maia S,Castañeda J,Omoto Y,Kim HJ,Warner M,Gustafsson JÅ

Tue Apr 21 00:00:00 EDT 2015

DACH1 is a novel predictive and prognostic biomarker in hepatocellular carcinoma as a negative regulator of Wnt/β-catenin signaling.

Oncotarget

Liu Y,Zhou R,Yuan X,Han N,Zhou S,Xu H,Guo M,Yu S,Zhang C,Yin T,Wu K

Mon Apr 20 00:00:00 EDT 2015

DACH1 is a novel predictive and prognostic biomarker in hepatocellular carcinoma as a negative regulator of Wnt/β-catenin signaling.

Oncotarget

Liu Y,Zhou R,Yuan X,Han N,Zhou S,Xu H,Guo M,Yu S,Zhang C,Yin T,Wu K

Mon Apr 20 00:00:00 EDT 2015

DACH1 inhibits lung adenocarcinoma invasion and tumor growth by repressing CXCL5 signaling.

Oncotarget

Han N,Yuan X,Wu H,Xu H,Chu Q,Guo M,Yu S,Chen Y,Wu K

Fri Mar 20 00:00:00 EDT 2015

Androgen therapy reverses injuries caused by ethanol consumption in the prostate: testosterone as a possible target to ethanol-related disorders.

Life sciences

Mendes LO,Scarano WR,Rochel-Maia SS,Fioruci-Fontaneli BA,Chuffa LG,Anselmo-Franci JA,Martinez FE

Thu Jan 01 00:00:00 EST 2015

Androgen therapy reverses injuries caused by ethanol consumption in the prostate: testosterone as a possible target to ethanol-related disorders.

Life sciences

Mendes LO,Scarano WR,Rochel-Maia SS,Fioruci-Fontaneli BA,Chuffa LG,Anselmo-Franci JA,Martinez FE

Thu Jan 01 00:00:00 EST 2015

Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling.

Journal of cellular and molecular medicine

Yan W,Wu K,Herman JG,Brock MV,Zhou Y,Lu Y,Zhang Z,Yang Y,Guo M

Mon Dec 01 00:00:00 EST 2014

Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling.

Journal of cellular and molecular medicine

Yan W,Wu K,Herman JG,Brock MV,Zhou Y,Lu Y,Zhang Z,Yang Y,Guo M

Mon Dec 01 00:00:00 EST 2014

The sinus venosus contributes to coronary vasculature through VEGFC-stimulated angiogenesis.

Development (Cambridge, England)

Chen HI,Sharma B,Akerberg BN,Numi HJ,Kivelä R,Saharinen P,Aghajanian H,McKay AS,Bogard PE,Chang AH,Jacobs AH,Epstein JA,Stankunas K,Alitalo K,Red-Horse K

Mon Dec 01 00:00:00 EST 2014

DACH1 inhibits cyclin D1 expression, cellular proliferation and tumor growth of renal cancer cells.

Journal of hematology & oncology

Chu Q,Han N,Yuan X,Nie X,Wu H,Chen Y,Guo M,Yu S,Wu K

Fri Oct 17 00:00:00 EDT 2014

DACH1 inhibits cyclin D1 expression, cellular proliferation and tumor growth of renal cancer cells.

Journal of hematology & oncology

Chu Q,Han N,Yuan X,Nie X,Wu H,Chen Y,Guo M,Yu S,Wu K

Fri Oct 17 00:00:00 EDT 2014

Auto-attraction of neural precursors and their neuronal progeny impairs neuronal migration.

Nature neuroscience

Ladewig J,Koch P,Brüstle O

Wed Jan 01 00:00:00 EST 2014

Specification of functional cranial placode derivatives from human pluripotent stem cells.

Cell reports

Dincer Z,Piao J,Niu L,Ganat Y,Kriks S,Zimmer B,Shi SH,Tabar V,Studer L

Thu Dec 12 00:00:00 EST 2013

Epigenetic regulation of DACH1, a novel Wnt signaling component in colorectal cancer.

Epigenetics

Yan W,Wu K,Herman JG,Brock MV,Fuks F,Yang L,Zhu H,Li Y,Yang Y,Guo M

Sun Dec 01 00:00:00 EST 2013

Stem cells expanded from the human embryonic hindbrain stably retain regional specification and high neurogenic potency.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tailor J,Kittappa R,Leto K,Gates M,Borel M,Paulsen O,Spitzer S,Karadottir RT,Rossi F,Falk A,Smith A

Wed Jul 24 00:00:00 EDT 2013

MicroRNA-195 targets ADP-ribosylation factor-like protein 2 to induce apoptosis in human embryonic stem cell-derived neural progenitor cells.

Cell death & disease

Zhou Y,Jiang H,Gu J,Tang Y,Shen N,Jin Y

Thu Jun 27 00:00:00 EDT 2013

Dachshund binds p53 to block the growth of lung adenocarcinoma cells.

Cancer research

Chen K,Wu K,Cai S,Zhang W,Zhou J,Wang J,Ertel A,Li Z,Rui H,Quong A,Lisanti MP,Tozeren A,Tanes C,Addya S,Gormley M,Wang C,McMahon SB,Pestell RG

Sat Jun 01 00:00:00 EDT 2013

Dachshund binds p53 to block the growth of lung adenocarcinoma cells.

Cancer research

Chen K,Wu K,Cai S,Zhang W,Zhou J,Wang J,Ertel A,Li Z,Rui H,Quong A,Lisanti MP,Tozeren A,Tanes C,Addya S,Gormley M,Wang C,McMahon SB,Pestell RG

Sat Jun 01 00:00:00 EDT 2013

Embryonic stem cell-based modeling of tau pathology in human neurons.

The American journal of pathology

Mertens J,Stüber K,Poppe D,Doerr J,Ladewig J,Brüstle O,Koch P

Wed May 01 00:00:00 EDT 2013

Excitation-induced ataxin-3 aggregation in neurons from patients with Machado-Joseph disease.

Nature

Koch P,Breuer P,Peitz M,Jungverdorben J,Kesavan J,Poppe D,Doerr J,Ladewig J,Mertens J,Tüting T,Hoffmann P,Klockgether T,Evert BO,Wüllner U,Brüstle O

Wed Nov 23 00:00:00 EST 2011

Dachshund homologues play a conserved role in islet cell development.

Developmental biology

Kalousova A,Mavropoulos A,Adams BA,Nekrep N,Li Z,Krauss S,Stainier DY,German MS

Wed Dec 15 00:00:00 EST 2010

Dachshund homologues play a conserved role in islet cell development.

Developmental biology

Kalousova A,Mavropoulos A,Adams BA,Nekrep N,Li Z,Krauss S,Stainier DY,German MS

Wed Dec 15 00:00:00 EST 2010

A rosette-type, self-renewing human ES cell-derived neural stem cell with potential for in vitro instruction and synaptic integration.

Proceedings of the National Academy of Sciences of the United States of America

Koch P,Opitz T,Steinbeck JA,Ladewig J,Brüstle O

Tue Mar 03 00:00:00 EST 2009

Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.

Genes & development

Elkabetz Y,Panagiotakos G,Al Shamy G,Socci ND,Tabar V,Studer L

Tue Jan 15 00:00:00 EST 2008

Bioinformatics

Protein Aliases: dac homolog; Dach1; dachshund family transcription factor 1; Dachshund homolog 1; FLJ10138; OTTHUMP00000040839; RP11-512J14.1

Gene Aliases: Dac; DACH; DACH1

UniProt ID: (Human) Q9UI36, (Mouse) Q9QYB2

Entrez Gene ID: (Human) 1602, (Rat) 306096, (Mouse) 13134

Molecular Function: winged helix/forkhead transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter suckling behavior multicellular organism development respiratory gaseous exchange cell proliferation negative regulation of transcription by competitive promoter binding negative regulation of cell migration regulation of nuclear cell cycle DNA replication negative regulation of transcription, DNA-templated development of primary female sexual characteristics negative regulation of fibroblast proliferation RNA polymerase II transcriptional preinitiation complex assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of DNA biosynthetic process regulation of transcription, DNA-templated transcription, DNA-templated transcription from RNA polymerase II promoter

Process(es)

transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding protein binding transcription factor activity, sequence-specific DNA binding

DACH1 Polyclonal Antibody

Product Details

10914-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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