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Rockland
DFF40 Polyclonal Antibody
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FIGURE: 1 / 2
DFF40 Antibody (600-401-AV3) in ICC/IF
Immunocytochemistry of DFF40 antibody. Cells: K562 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: DFF40 antibody at 10 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DFF40 is nuclear and cytoplasmic. Staining: DFF40 as precipitated brown signal with hematoxylin blue nuclear counterstain.
Product Details
600-401-AV3
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-DFF40 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the internal region of human DFF40.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis which is a cell death process removing toxic and/or useless cells during mammalian development can be induced by a variety of stimuli, including the death factors of the tumor necrosis factor family, or UV irradiation, anticancer drugs, and survival factor deprivation. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA. Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Currently, fourteen members of the caspase family have been identified, of which seven mediate apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2410008J01Rik; AU018859; CAD; Caspase-activated deoxyribonuclease; caspase-activated DNase; Caspase-activated nuclease; Cpad; CPAN; DFF-2; DFF-40; DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase); DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, 40 kD, beta subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta subunit; DNase inhibited by DNA fragmentation factor; RP13-531C17.1; RP23-254N4.2
Gene Aliases: 40kDa; 5730477D02Rik; CAD; CPAN; DFF-40; DFF2; DFF40; DFFB; Didff
UniProt ID: (Human) O76075, (Rat) Q99N34, (Mouse) O54788
Entrez Gene ID: (Human) 1677, (Rat) 84359, (Mouse) 13368
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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