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DLL3 Antibody (PA5-26336) in ICC/IF
Immunocytochemistry analysis of DLL3 in U-2 OS (human osteosarcoma cell line) cells. Samples were incubated with DLL3 polyclonal antibody (Product # PA5-26336) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter sta... View More
Product Details
PA5-26336
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human DLL3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2543836
Target Information
Delta-like protein 3 (DLL3) is a ligand for the Notch signaling pathway. It inhibits primary neurogenesis. DLL3 plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Defects in DLL3 are the cause of Spondylocostal dysostosis autosomal recessive type 1 (SCDO1). Mutations in DLL3 gene cause truncal shortening relative to their limbs, which leads to abdominal protrusion, abnormal spinal curvature and sometimes a plagiocephaly-torticollis sequence. It may be required to divert neurons along a specific differentiation pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: delta-like 3; Delta-like protein 3; Delta3; Drosophila Delta homolog 3; M-Delta-3
Gene Aliases: DLL3; pu; pudgy; SCDO1
UniProt ID: (Human) Q9NYJ7, (Mouse) O88516, (Rat) O88671
Entrez Gene ID: (Human) 10683, (Mouse) 13389, (Rat) 114125
skeletal system development
in utero embryonic development
cell fate determination
somitogenesis
Notch signaling pathway
multicellular organism development
compartment pattern specification
nervous system development
tissue development
cell differentiation
negative regulation of Notch signaling pathway
paraxial mesoderm development
negative regulation of astrocyte differentiation
negative regulation of neurogenesis
positive regulation of neurogenesis
central nervous system development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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