Invitrogen

DLL3 Polyclonal Antibody

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DLL3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of 293 cells using a DLL3 polyclonal antibody (Product # PA5-26336) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

DLL3 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts of U-87 MG (Lane 1), SH-SY5Y (Lane 2), A549 (Lane 3), A-375 (Lane 4), HeLa (Lane 5), HEK-293 (Lane 6), tissue extracts of Mouse Pup Brain (Lane 7), Mouse Brain (Lane 8) and Rat Brain (Lane 9). The blot was probed with Anti-DLL3 Polyclonal Antibody (Product # PA5-26336, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 62 kDa band corresponding to DLL3 was observed across the cell lines and tissues tested.

Product Details

PA5-26336

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:25	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human DLL3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2543836

Target Information

Delta-like protein 3 (DLL3) is a ligand for the Notch signaling pathway. It inhibits primary neurogenesis. DLL3 plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Defects in DLL3 are the cause of Spondylocostal dysostosis autosomal recessive type 1 (SCDO1). Mutations in DLL3 gene cause truncal shortening relative to their limbs, which leads to abdominal protrusion, abnormal spinal curvature and sometimes a plagiocephaly-torticollis sequence. It may be required to divert neurons along a specific differentiation pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparison of four DLL3 antibodies performance in high grade neuroendocrine lung tumor samples and cell cultures.

Diagnostic pathology

Brcic L,Kuchler C,Eidenhammer S,Pabst D,Quehenberger F,Gazdar AF,Popper H

PA5-26336 was used in Immunohistochemistry to suggest evidence of DLL3 expression in high proportions of patients with LCNEC might provide basis for studies of new therapy options in this group of patients.