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Invitrogen
ECM29 Polyclonal Antibody
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FIGURE: 1 / 5
ECM29 Antibody (PA3-035) in ICC/IF
Immunofluorescent analysis of Ecm29 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ecm29 Rabbit Polyclonal Antibody (Product # PA3-035) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produc... View More
Product Details
PA3-035
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-035 detects Ecm29 in human and mouse samples.
PA3-035 has been used successfully in Western blot and immunofluorescence procedures. In Western blot analysis of HeLa cell extracts this antibody detects a ~205 kDa protein representing Ecm29.
The PA3-035 immunogen is a synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.
Target Information
Ecm29 appears to be one of three proteins which associate significantly with proteasomes. Ecm29 has been shown to enhance the stability of the proteasome. Ecm29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that Ecm29 tethers the regulatory complex to the proteasome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ecm29; ECM29 homolog, proteasome accessory protein; Ecm29 proteasome adapter and scaffold; FLJ22036; homolog of yeast Ecm29; KIAA0368; KIAA1962; Proteasome adapter and scaffold protein ECM29; Proteasome-associated protein ECM29 homolog; RP11-386D8.2; unnamed protein product
Gene Aliases: AW558785; BB181316; ECM29; ECPAS; KIAA0368; mKIAA0368
UniProt ID: (Mouse) Q6PDI5, (Human) Q5VYK3
Entrez Gene ID: (Mouse) 230249, (Human) 23392
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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