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Invitrogen
EED Polyclonal Antibody
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FIGURE: 1 / 10
EED Antibody (PA5-34430) in ICC/IF
Immunofluorescence analysis of EED was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EED Rabbit Polyclonal Antibody (Product # PA5-34430) at 20 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
Product Details
PA5-34430
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
An 18 amino acid synthetic peptide near the amino terminus of human EED
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is human heart tissue lysate.
PA5-34430 can be used with blocking peptide PEP-1472.
Target Information
The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin Beta-7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Embryonic ectoderm development protein; hEED; lethal, Chr 7, Rinchik 5; Polycomb protein EED; WAIT-1; WD protein associating with integrin cytoplasmic tails 1
Gene Aliases: EED; ENSMUSG00000039373; HEED; l(7)5Rn; l7Rn5; lusk; WAIT1
UniProt ID: (Human) O75530, (Mouse) Q921E6
Entrez Gene ID: (Human) 8726, (Mouse) 13626, (Rat) 293104
negative regulation of transcription from RNA polymerase II promoter
regulation of gene expression by genetic imprinting
transcription, DNA-templated
histone methylation
spinal cord development
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of histone H3-K27 methylation
regulation of transcription, DNA-templated
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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