Proteintech

ENO3 Polyclonal Antibody

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ENO3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ENO3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).

ENO3 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

55234-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.76 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.

Target Information

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ENO3 promotes colorectal cancer progression by enhancing cell glycolysis.

Medical oncology (Northwood, London, England)

Chen J,Zhang Z,Ni J,Sun J,Ju F,Wang Z,Wang L,Xue M

Thu Apr 28 00:00:00 EDT 2022

ENO3 promotes colorectal cancer progression by enhancing cell glycolysis.

Medical oncology (Northwood, London, England)

Chen J,Zhang Z,Ni J,Sun J,Ju F,Wang Z,Wang L,Xue M

Thu Apr 28 00:00:00 EDT 2022

Characterization of the liver proteome in dairy cows experiencing negative energy balance at early lactation.

Journal of proteomics

Swartz TH,Moallem U,Kamer H,Kra G,Levin Y,Mamedova LK,Bradford BJ,Zachut M

Mon Aug 30 00:00:00 EDT 2021

ENO3 promoted the progression of NASH by negatively regulating ferroptosis via elevation of GPX4 expression and lipid accumulation.

Annals of translational medicine

Lu D,Xia Q,Yang Z,Gao S,Sun S,Luo X,Li Z,Zhang X,Han S,Li X,Cao M

Thu Apr 01 00:00:00 EDT 2021

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca²⁺ ATPase isoform in sturgeon sperm cryopreservation.

Reproduction in domestic animals = Zuchthygiene

Xi MD,Li P,Du H,Qiao XM,Liu ZG,Wei QW

Sun Apr 01 00:00:00 EDT 2018

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca²⁺ ATPase isoform in sturgeon sperm cryopreservation.

Reproduction in domestic animals = Zuchthygiene

Xi MD,Li P,Du H,Qiao XM,Liu ZG,Wei QW

Sun Apr 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Beta-enolase; enob; Enolase 3; enolase 3 (beta, muscle); MSE; muscle enriched enolase; Muscle specific enolase (beta beta enolose) (two splicing products); Muscle-specific enolase; Skeletal muscle enolase

Gene Aliases: BBE; Eno-3; ENO3; GSD13; MSE

UniProt ID: (Human) P13929, (Mouse) P21550, (Rat) P15429

Entrez Gene ID: (Human) 2027, (Mouse) 13808, (Rat) 25438

Molecular Function: lyase